概述

  • 产品名称

  • 描述

    兔多克隆抗体to CD31
  • 宿主

    Rabbit
  • 特异性

    Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries.
  • 经测试应用

    适用于: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性

    与反应: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q08481

  • 阳性对照

    • IHC-P: Mouse skin, xenograft tissue; Human tonsil tissue. WB: Jurkat whole cell lysate (ab7899), Human vascular endothelial cell lysate. IHC-Fr: Human fetal heart tissue.
  • 常规说明

      

应用

Our Abpromise guarantee covers the use of ab28364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/20. PubMed: 17316380
IHC-FoFr Use at an assay dependent concentration.
WB 1/500.

靶标

  • 功能

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 数据库链接

  • 别名

    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

图片

  • CLARITY-based immunochemistry showing Nerve fibers and vessels in the mesentery.

    A) Catecholaminergic nerve fibers stained for TH. (B) Nerves stained for the pan-neuronal marker TUJ-1. (A,B) Illustrate the integration of nerve fibers in the mesentery (asterisk) into the intramural neuronal networks of the gut. (C) Depicts blood vessels stained with the endothelial marker CD31 (using ab28364 at 1:50) and shows the branching of vessels coming from the mesentery (asterisk) and fine capillaries in the villi (confocal image stack 10x/0.3 objective, z-range 1047 μm). (D–F) Show the innervation of blood vessels in the mesentery ((D) CD31 (using ab28364 at 1:50), (E) TUJ-1, (F) Merge). (G–I) Identifies the very fine sympathetic nerve fibers of arterioles by specific catecholaminergic staining. Scale bars: (A–C) 500 μm; (D–F) 100 μm; (G–I) 100 μm.

    Displaying the mesentery and intact gut in one preparation is a major improvement in visualizing the neuronal and vascular interface between the alimentary tract and its extrinsic supply, rendering CLARITY-based immunochemistry a promising tool going beyond previous whole mount preparations.

    D can be visualized  Supplementary video 6 to fully appreciate the three-dimensional tissue composition.

  • ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ab28364 at a 1/300 dilution, staining human CD31 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/50 dilution staining mouse tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was formaldehyde fixed and blocked with serum. The tissue was incubated with the primary antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary. The endothelium of vessels of any tissues are positive.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/500 dilution + Human vascular endothelial cells at 25 µg

    Secondary
    anti-rabbit HRP at 1/5000 dilution

    See Abreview

  • Representative images of cross sections with CD31 staining in red, nucleus in blue. White dotted lines indicate the graft wall. Scale bar = 100 μm.

    Explanted grafts from C57/BL6 mice were fixed overnight in paraformaldehyde (2%) at room temperature. Samples were dehydrated through an ethanol gradient and embedded in paraffin and sectioned at 5 μm transversely from proximal anastomosis to distal anastomosis. For immunohistochemistry staining, sections were deparaffinised and stained with antibody against CD31 (Abcam, ab28364, 1:200) for endothelial cells.

     

  • ab28364 staining CD31 in mouse xenograft tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices and blocked with 1% BSA for 60 minutes at 21°C. Antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. The sample was incubated with primary antibody (20 ug/mL) at 4°C for 16 hours. A HRP-conjugated goat anti-rabbit polyclonal (2 ug/mL) was used as the secondary antibody.

    See Abreview

  • GFP positive cells (green) were colocalized with CD31 (red). Nuclear staining with DAPI is blue.

    For immunofluorescence staining, the skin samples were fixed in 4% paraformaldehyde and embedded in paraffin. Skin sections were stained with an anti-GFP antibody. The skin sections were also treated with primary Abs against CD31 (Abcam, ab28364, diluted 1:300). Secondary antibodies conjugated to rhodamine-isothiocyanate were used for fluorescence detection on a confocal laser scanning fluorescence microscope (Zeiss, German).

    Opn-/-; OPN (Osteopontin) knock-out mice.

文献

This product has been referenced in:

  • Xu L  et al. ATM deficiency promotes progression of CRPC by enhancing Warburg effect. Endocr Relat Cancer 26:59-71 (2019). Read more (PubMed: 30400006) »
  • Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). Read more (PubMed: 30412364) »
See all 948 Publications for this product

客户评价及客户问答

31-40 of 155 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (skeletal muscle)
Permeabilization
No
Specification
skeletal muscle
Blocking step
universal blocking reagent (Biogenex) as blocking agent for 8 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Aug 23 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Left ventricle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trilogy
Permeabilization
Yes - Trilogy
Specification
Left ventricle
Blocking step
Dako Envision Kit 4007 (Block) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
10% Formalin

Abcam user community

Verified customer

提交于 Mar 16 2017

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain

Abcam user community

Verified customer

提交于 Feb 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Jan 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization
No
Specification
Brain
Fixative
Formalin 4%

Mr. Emmanuel Bouchaert

Verified customer

提交于 Jan 16 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (aorta)
Permeabilization
No
Specification
aorta
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Dec 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

提交于 Aug 26 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

提交于 Aug 23 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (stomach)
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone

Abcam user community

Verified customer

提交于 Aug 22 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH8
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

提交于 Aug 09 2016

31-40 of 155 Abreviews or Q&A

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