概述

  • 产品名称

  • 描述

    兔多克隆抗体to CD31
  • 宿主

    Rabbit
  • 特异性

    Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries.
  • 经测试应用

    适用于: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性

    与反应: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q08481

  • 阳性对照

    • IHC-P: Mouse skin, xenograft tissue; Human tonsil tissue. WB: Jurkat whole cell lysate (ab7899), Human vascular endothelial cell lysate. IHC-Fr: Human fetal heart tissue.
  • 常规说明

      

应用

Our Abpromise guarantee covers the use of ab28364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/20. PubMed: 17316380
IHC-FoFr Use at an assay dependent concentration.
WB 1/500.

靶标

  • 功能

    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性

    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性

    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域

    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰

    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位

    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 数据库链接

  • 别名

    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

图片

  • CLARITY-based immunochemistry showing Nerve fibers and vessels in the mesentery.

    A) Catecholaminergic nerve fibers stained for TH. (B) Nerves stained for the pan-neuronal marker TUJ-1. (A,B) Illustrate the integration of nerve fibers in the mesentery (asterisk) into the intramural neuronal networks of the gut. (C) Depicts blood vessels stained with the endothelial marker CD31 (using ab28364 at 1:50) and shows the branching of vessels coming from the mesentery (asterisk) and fine capillaries in the villi (confocal image stack 10x/0.3 objective, z-range 1047 μm). (D–F) Show the innervation of blood vessels in the mesentery ((D) CD31 (using ab28364 at 1:50), (E) TUJ-1, (F) Merge). (G–I) Identifies the very fine sympathetic nerve fibers of arterioles by specific catecholaminergic staining. Scale bars: (A–C) 500 μm; (D–F) 100 μm; (G–I) 100 μm.

    Displaying the mesentery and intact gut in one preparation is a major improvement in visualizing the neuronal and vascular interface between the alimentary tract and its extrinsic supply, rendering CLARITY-based immunochemistry a promising tool going beyond previous whole mount preparations.

    D can be visualized  Supplementary video 6 to fully appreciate the three-dimensional tissue composition.

  • ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ab28364 at a 1/300 dilution, staining human CD31 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/50 dilution staining mouse tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was formaldehyde fixed and blocked with serum. The tissue was incubated with the primary antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary. The endothelium of vessels of any tissues are positive.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/500 dilution + Human vascular endothelial cells at 25 µg

    Secondary
    anti-rabbit HRP at 1/5000 dilution

    See Abreview

  • Representative images of cross sections with CD31 staining in red, nucleus in blue. White dotted lines indicate the graft wall. Scale bar = 100 μm.

    Explanted grafts from C57/BL6 mice were fixed overnight in paraformaldehyde (2%) at room temperature. Samples were dehydrated through an ethanol gradient and embedded in paraffin and sectioned at 5 μm transversely from proximal anastomosis to distal anastomosis. For immunohistochemistry staining, sections were deparaffinised and stained with antibody against CD31 (Abcam, ab28364, 1:200) for endothelial cells.

     

  • ab28364 staining CD31 in mouse xenograft tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices and blocked with 1% BSA for 60 minutes at 21°C. Antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. The sample was incubated with primary antibody (20 ug/mL) at 4°C for 16 hours. A HRP-conjugated goat anti-rabbit polyclonal (2 ug/mL) was used as the secondary antibody.

    See Abreview

  • GFP positive cells (green) were colocalized with CD31 (red). Nuclear staining with DAPI is blue.

    For immunofluorescence staining, the skin samples were fixed in 4% paraformaldehyde and embedded in paraffin. Skin sections were stained with an anti-GFP antibody. The skin sections were also treated with primary Abs against CD31 (Abcam, ab28364, diluted 1:300). Secondary antibodies conjugated to rhodamine-isothiocyanate were used for fluorescence detection on a confocal laser scanning fluorescence microscope (Zeiss, German).

    Opn-/-; OPN (Osteopontin) knock-out mice.

文献

This product has been referenced in:

  • Xu L  et al. ATM deficiency promotes progression of CRPC by enhancing Warburg effect. Endocr Relat Cancer 26:59-71 (2019). Read more (PubMed: 30400006) »
  • Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). Read more (PubMed: 30412364) »
See all 988 Publications for this product

客户评价及客户问答

121-130 of 160 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100 in PBS
Blocking step
No blocking step used for 30 minute(s) · Concentration: 5 · Temperature: 25°C

Ms. Kristine Jun

Verified customer

提交于 Mar 16 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Blood Vessels in White Adipose Tissue)
Specification
Blood Vessels in White Adipose Tissue
Fixative
4% PFA for 10 minutes at room temp, no antigen retrieval
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Mar 13 2012

Question
Answer

I checked the sequence homology between salmon CD31 and the immunogen of ab28364, and the homology is only a few percent. Because of this, we would not believe that ab28364 would cross react with salmon samples.

Read More

Answer

Please find my answers to your questions below:

1. I recommend to dilute antibodies in PBS or TBS instead of water.

2. The best storage option for antibodies is un-diluted. I suggest to only prepare the amount of diluted antibody that is necessary for one experiment and use it up immediately.

3. If an antibody has to be stored frozen, I strongly recommend to prepare aliquots and only to defrost as much as is needed for one experiment. If the aliquot contains more than that is can be keep at 4C for a couple of days.
As a general guideline, HRP conjugated antibodies should be stored at 4C.
We guarantee our antibodies to work as stated on the datasheet only when stored as instructed on the datasheet.

4. We do have an extensive protocols section on our homepage:

https://www.abcam.com/index.html?pageconfig=popular_protocols

including protocols for

antibody storage:

https://www.abcam.com/index.html?pageconfig=resource&rid=10795

and IHC

https://www.abcam.com/index.html?pageconfig=resource&rid=11384

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

Read More

Answer

I would recommend the following kits to you:
Streptavidin Alkaline Phosphatase (Ready to Use), www.abcam.com/ab64268
Streptavidin Peroxidase (Ready to Use), www.abcam.com/ab64269

Read More

Answer

I would strongly recommend performing antigen retrieval with citrate buffer pH 6 before commencing with the staining. Unmasking the epitope is necessary for the antibody to bind.


You can find our recommended protocol here:
https://www.abcam.com/index.html pageconfig=resource&rid=11488

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ctrate buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Jan 11 2012

Answer

We haven't specifically tested this antibody with bone, however we do usually recommend a few resources to customers when working with this tricky tissue type. The trick is to glue the section down and section adhesion really is key.
http://www.ihcworld.com/_technical_tips/prevent_section_fall.htm
And I always recommend searching this bulletin board archives. Use "bone" and "retrieval" or "AR" as keywords:
http://www.histosearch.com/histonet.html
And I know you said you're analyzing marrow and here are some references: http://www.ncbi.nlm.nih.gov/pubmed/8714271
http://jhc.sagepub.com/content/40/6/787.short
http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1096-9896(199709)183:1%3C116::AID-PATH1087%3E3.0.CO;2-2/abstract

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain)
Specification
mouse brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M Tris pH 10
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Jan 04 2012

Answer

I very strongly recommend using a permeablization step in your protocol. I would recommend using 0.1% or 0.2% Triton or NP-40 for 10m minutes only at room temperature. Other customers have included this step in their protocols when working with mouse tissue with good results. If you care to see those, they have posted them on the product page under the Abreviews tab. I've included the link for you here: https://www.abcam.com/CD31-antibody-ab28364-reviews.html

Read More

121-130 of 160 Abreviews or Q&A

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