Anti-CD31抗体(ab28364)
Key features and details
- Rabbit polyclonal to CD31
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
重组抗体的批间可重复性更强
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CD31抗体
参阅全部 CD31 一抗 -
描述
兔多克隆抗体to CD31 -
宿主
Rabbit -
特异性
Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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免疫原
Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q08481 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| IHC-P |
Human
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| All applications |
Mouse
Pig
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (84) |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue |
| 说明 |
|---|
|
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples. Positive Control: Hu tonsil tissue |
靶标
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功能
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
组织特异性
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
序列相似性
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
结构域
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
翻译后修饰
Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
细胞定位
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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数据库链接
- Entrez Gene: 5175 Human
- Entrez Gene: 18613 Mouse
- Omim: 173445 Human
- SwissProt: P16284 Human
- SwissProt: Q08481 Mouse
- Unigene: 376675 Human
- Unigene: 514412 Human
- Unigene: 343951 Mouse
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别名
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)This image is courtesy of an Abreview submitted by Mr. Victor Bernal.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (30 minutes at 23°C). 10% Neutral buffered formalin used as fixative. Heat mediated antigen retrieval was performed at 95°C for 20 minutes. Blocking performed using 2.5% serum for 20 minutes at 23°C. HRP-conjugated Goat Anti-rabbit was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)This image is courtesy of an anonymous Abreview.Immunohistochemistry (PFA-fixed paraffin-embedded sections) analysis of human palatine tonsil tissue labelling CD31 with ab28364 at 1/50 dilution (15 hours at 4°C). Heat mediated antigen retrieval was performed using Tris/EDTA buffer. Blocking performed with 5% normal goat serum for 1 hour at 21°C. HRP-conjugated Goat Anti-Rabbit was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (ab28364)ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1173)
ab28364 被引用在 1173 文献中.
- Stock AA et al. Conformal Coating of Stem Cell-Derived Islets for ß Cell Replacement in Type 1 Diabetes. Stem Cell Reports 14:91-104 (2020). PubMed: 31839542
- Barbeito-Andrés J et al. Congenital Zika syndrome is associated with maternal protein malnutrition. Sci Adv 6:eaaw6284 (2020). PubMed: 31950075
- Ayanlaja AA et al. Doublecortin undergo nucleocytoplasmic transport via the RanGTPase signaling to promote glioma progression. Cell Commun Signal 18:24 (2020). PubMed: 32050972
- Rutkai I et al. Chronic imaging of mitochondria in the murine cerebral vasculature using in vivo two-photon microscopy. Am J Physiol Heart Circ Physiol 318:H1379-H1386 (2020). PubMed: 32330090
- Luo S et al. Targeting self-assembly peptide for inhibiting breast tumor progression and metastasis. Biomaterials 249:120055 (2020). PubMed: 32315863
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com