Key features and details
- Mouse monoclonal [Ber-H2] to CD30, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
参阅全部 CD30 一抗
描述小鼠单克隆抗体[Ber-H2] to CD30，prediluted
特异性This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.
经测试应用适用于: IHC-Pmore details
BALB/C mice were injected with L428 cell line cells.
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Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
存储溶液Preservative: 0.05% Sodium azide
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Our Abpromise guarantee covers the use of ab871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes may increase staining intensity.
Although not required, microwave treatment will considerably enhance the signal.
功能Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.
序列相似性Contains 6 TNFR-Cys repeats.
翻译后修饰Phosphorylated on serine and tyrosine residues.
细胞定位Cytoplasm and Cell membrane.
- Information by UniProt
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab871 被引用在 1 文献中.
- Keren L et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026