Anti-CD3抗体[PS1] (ab699)
概述
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产品名称Anti-CD3抗体[PS1]
参阅全部 CD3 一抗 -
描述小鼠单克隆抗体[PS1] to CD3
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宿主Mouse
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特异性This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells.
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经测试应用适用于: IHC-P, IHC-Fr, WB, ICC/IFmore details
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种属反应性与反应: Human
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免疫原
Prokaryotic fusion protein corresponding to the epsilon chain of CD3
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阳性对照
- Tonsil or T-cell lymphoma. IHC-P: Human colon tissue and colorectal mucosa tissue. IHC-Fr: Human skin tissue.
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常规说明This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: Proprietary, not sodium azide or thimerosal.
Constituents: PBS, Tissue Culture Supernatant. pH 7.3, Protein carrier. -
Concentration information loading... -
克隆单克隆
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克隆编号PS1
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骨髓瘤unknown
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同种型IgG2a
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轻链类型unknown
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研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
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Unmasking reagent
应用
Our Abpromise guarantee covers the use of ab699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. PubMed: 21654633 | |
| ICC/IF | Use at an assay dependent concentration. |
靶标
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功能The CD3 complex mediates signal transduction.
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疾病相关Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
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序列相似性Contains 1 ITAM domain.
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细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
别名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.cn/ps/products/0/ab699/Images/CD3-Primary-antibodies-ab699-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [PS1] (ab699)This image is courtesy of an anonymous Abreviewab699 staining CD3 in paraffin-embedded human colorectal mucosa tissue sections by IHC-P. Tissue was fixed with formaldehyde and blocked with 10% Goat serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/50 in PBS/BSA/Tween) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti mouse/rabbit polymer was used as secondary antibody.
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![Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.cn/ps/products/0/ab699/Images/CD3-Primary-antibodies-ab699-15.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)Image courtesy of an anonymous Abreview.ab699 staining CD3 in human colon tissue by immunohistochemistry. Tissue was fixed in acetone, blocked using 10% donkey serum/PBS for 30 minutes at 21°C and then incubated with ab699 at a 1/40 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor® 555 conjugated donkey anti-mouse polyclonal used at a 1/1000 dilution. CD3 in red nuclear counterstaining with DAPI in blue.
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![Western blot - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.cn/ps/products/0/ab699/Images/ab699-282859-ab69955.jpg)
Western blot - Anti-CD3 antibody [PS1] (ab699)All lanes : Anti-CD3 antibody [PS1] (ab699) at 1/1000 dilution
Lane 1 : Positive control - CD3 protein
Lane 2 : Whole cell Jurkat lysate
Lane 3 : Whole cell PBMCs lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat polyclonal anti-mouse CD3 HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 minutesGel running conditions: Reduced non-denaturing (native) (5-16% gels).
Incubation time: 16 hours at 4°C in 5% BSA/ TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.cn/ps/products/0/ab699/Images/ab699-282860-ab69956.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [PS1] (ab699)Methanol fixed human lymphocyte cells stained for CD3 (red) using ab699 at a 1/75 dilution in ICC/IF. Blocked for 30 minutes at RT using 4% BSA. Incubation time: 16 hours at 4°C in PBS/4% BSA. Secondary antibody: Goat polyclonal anti-mouse TexasRed® at a 1/100 dilution.
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![Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.cn/ps/products/0/ab699/Images/CD3-Primary-antibodies-ab699-16.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)Image courtesy of an anonymous Abreview.ab669 staining CD3 in human skin tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in methanol, blocked using 4% BSA for 30 minutes at room temperature and then incubated with ab699 at a 1/75 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-mouse polyclonal used at 10µg/ml.
实验方案
文献
This product has been referenced in:
- Liu F et al. Increased T-helper 17 cell differentiation mediated by exosome-mediated microRNA-451 redistribution in gastric cancer infiltrated T cells. Cancer Sci 109:65-73 (2018). ICC/IF . Read more (PubMed: 29059496) »
- van Balen P et al. Tissue Damage Caused by Myeloablative, but Not Non-Myeloablative, Conditioning before Allogeneic Stem Cell Transplantation Results in Dermal Macrophage Recruitment without Active T-Cell Interaction. Front Immunol 9:331 (2018). Read more (PubMed: 29535719) »
