Key features and details
- Rabbit polyclonal to CD3
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG
参阅全部 CD3 一抗
ab828 is predicted to react with T cell areas in lymphoid tissues derived from various species: human, bovine, porcine, sheep, cat, dog, rat, mouse, Psammomys (sand rat), and chicken. The molecular weight of the polypeptide recognized by the antibody in bovine and swine extracts is 20 kDa.
经测试应用适用于: IHC-P, IHC-Frmore details
Synthetic peptide corresponding to Human CD3 conjugated to Bovine Serum Albumin (BSA).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/10 - 1/50. ABC method.|
功能The CD3 complex mediates signal transduction.
疾病相关Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
序列相似性Contains 1 ITAM domain.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
This immunoperoxidase picture shows inflammatory cell infiltration with many T-lymphocytes in the interstitium of human kidneys with congenital nephrotic syndrome of the Finnish type (CNF, NPHS1). Immunoperoxidase staining of frozen sections with ab828 antibody (oval shaped, unstained spaces on the left are glomeruli).
This image was kindly supplied as part of the review submitted by Arvi-Matti-Kuusniemi.
Ab828 positively staining formaldehyde fixed human tonsil tissue at 1/50 in conjunction with goat anti rabbit HRP. Detection by DAB.
Pressure cooker heat mediated antigen retrieval was employed in citrate buffer pH 6.0.
This image is courtesy of an Abreview submitted by Melanie Black on 22 Aug 05. We do not have any further information relating to this image.
IHC-P image of CD3 staining on rat small intestine using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
IHC-P image of CD3 staining on monkey colon using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
IHC-P image of CD3 staining on dog spleen using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
ab828 被引用在 40 文献中.
- Yoon SW et al. Locally Applied Slow-Release of Minocycline Microspheres in the Treatment of Peri-Implant Mucositis: An Experimental In Vivo Study. Pharmaceutics 12:N/A (2020). PubMed: 32708741
- Souri Z et al. Loss of BAP1 Is Associated with Upregulation of the NFkB Pathway and Increased HLA Class I Expression in Uveal Melanoma. Cancers (Basel) 11:N/A (2019). PubMed: 31382450
- AbuAli AM et al. Cellular elements in the developing caecum of Japanese quail (Coturnix coturnix japonica): morphological, morphometrical, immunohistochemical and electron-microscopic studies. Sci Rep 9:16241 (2019). PubMed: 31700017
- Sablik KA et al. Immune Cell Infiltrate in Chronic-Active Antibody-Mediated Rejection. Front Immunol 10:3106 (2019). PubMed: 32117198
- Heidt S et al. Presence of intragraft B cells during acute renal allograft rejection is accompanied by changes in peripheral blood B cell subsets. Clin Exp Immunol N/A:N/A (2019). PubMed: 30712266