Anti-CD3抗体(ab828)
Key features and details
- Rabbit polyclonal to CD3
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Rat, Dog, Human, Common marmoset
- Isotype: IgG
概述
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产品名称
Anti-CD3抗体
参阅全部 CD3 一抗 -
描述
兔多克隆抗体to CD3 -
宿主
Rabbit -
特异性
ab828 is predicted to react with T cell areas in lymphoid tissues derived from various species: human, bovine, porcine, sheep, cat, dog, rat, mouse, Psammomys (sand rat), and chicken. The molecular weight of the polypeptide recognized by the antibody in bovine and swine extracts is 20 kDa.
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经测试应用
适用于: IHC-P, IHC-Frmore details -
种属反应性
与反应: Rat, Dog, Human, Common marmoset -
免疫原
Synthetic peptide corresponding to Human CD3 conjugated to Bovine Serum Albumin (BSA).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/50. PubMed: 1839075918390759 | |
| IHC-Fr | 1/10 - 1/50. ABC method. |
靶标
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功能
The CD3 complex mediates signal transduction. -
疾病相关
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
序列相似性
Contains 1 ITAM domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 25300 Rat
- Entrez Gene: 25710 Rat
- Entrez Gene: 300678 Rat
- Entrez Gene: 315609 Rat
see all -
别名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-CD3 antibody (ab828)This immunoperoxidase picture shows inflammatory cell infiltration with many T-lymphocytes in the interstitium of human kidneys with congenital nephrotic syndrome of the Finnish type (CNF, NPHS1). Immunoperoxidase staining of frozen sections with ab828 antibody (oval shaped, unstained spaces on the left are glomeruli).
This image was kindly supplied as part of the review submitted by Arvi-Matti-Kuusniemi. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)Ab828 positively staining formaldehyde fixed human tonsil tissue at 1/50 in conjunction with goat anti rabbit HRP. Detection by DAB.
Pressure cooker heat mediated antigen retrieval was employed in citrate buffer pH 6.0.
This image is courtesy of an Abreview submitted by Melanie Black on 22 Aug 05. We do not have any further information relating to this image.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of CD3 staining on rat small intestine using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of CD3 staining on monkey colon using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomIHC-P image of CD3 staining on dog spleen using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
数据表及文件
文献
ab828 被引用在 37 文献中.
- Heidt S et al. Presence of intragraft B cells during acute renal allograft rejection is accompanied by changes in peripheral blood B cell subsets. Clin Exp Immunol N/A:N/A (2019). PubMed: 30712266
- Souri Z et al. Loss of BAP1 Is Associated with Upregulation of the NFkB Pathway and Increased HLA Class I Expression in Uveal Melanoma. Cancers (Basel) 11:N/A (2019). PubMed: 31382450
- Bartolacci C et al. Phage-Based Anti-HER2 Vaccination Can Circumvent Immune Tolerance against Breast Cancer. Cancer Immunol Res 6:1486-1498 (2018). PubMed: 30327365
- Fiore A et al. Induction of immunosuppressive functions and NF-?B by FLIP in monocytes. Nat Commun 9:5193 (2018). PubMed: 30518925
- Dohmen AJC et al. Sponge-supported cultures of primary head and neck tumors for an optimized preclinical model. Oncotarget 9:25034-25047 (2018). PubMed: 29861851
