Application
IHC - Wholemount
Sample
Mouse Tissue (Oesophagus & Tongue tissue were stained, Tongue re)
Specification
Oesophagus & Tongue tissue were stained, Tongue re
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Blocking buffer (2% BSA, 10% Serum)
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Donkey a-Rabbit AF647+
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Tissues were fixed 30 mins in 4% PFA.
High background was observed, but some apparent positive cell staining does appear to be observed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 09 2021