重组Anti-CD27抗体[EPR8568] (ab133761)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8568] to CD27
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD27抗体[EPR8568]
参阅全部 CD27 一抗 -
描述
兔单克隆抗体[EPR8568] to CD27 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Raji and Ramos cell lysates; Ramos cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数(KD)
KD = 7.60 x 10 -13 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8568 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133761于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 29 kDa).
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ICC/IF |
1/50 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 29 kDa). |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. -
组织特异性
Found in most T-lymphocytes. -
序列相似性
Contains 3 TNFR-Cys repeats. -
翻译后修饰
Phosphorylated and O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 939 Human
- Entrez Gene: 500318 Rat
- Omim: 186711 Human
- SwissProt: P26842 Human
- Unigene: 355307 Human
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别名
- CD 27 antibody
- CD27 antibody
- CD27 antigen antibody
see all
图片
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All lanes : Anti-CD27 antibody [EPR8568] (ab133761) at 1/1000 dilution
Lane 1 : Wild-type Raji cell lysate
Lane 2 : CD27 knockout Raji cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : Human Brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesab133761 was shown to react with CD27 in wild-type Raji cells in western blot. Loss of signal was observed when CD27 knockout cell line ab274910 (knockout cell lysate ab281360) was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab133761 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1 in 5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.
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Immunofluorescent staining of CD27 in Ramos cells, using ab133761 at a 1/50 dilution.
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Anti-CD27 antibody [EPR8568] (ab133761) at 1/1000 dilution + Ramos cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 29 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab133761 尚未被引用在任何文献中。