概述

  • 产品名称
    Anti-CD22抗体[Myg13] (FITC)
    参阅全部 CD22 一抗
  • 描述
    小鼠单克隆抗体[Myg13] to CD22 (FITC)
  • 宿主
    Mouse
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 特异性
    This antibody does not react with plasma cells.
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human

性能

应用

Our Abpromise guarantee covers the use of ab1172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µl for 106 cells.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能
    Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • 组织特异性
    B-lymphocytes.
  • 序列相似性
    Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 结构域
    Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
  • 翻译后修饰
    Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
    Phosphorylated on tyrosine residues by LYN.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B cell receptor CD22 precursor antibody
    • B lymphocyte cell adhesion molecule antibody
    • B-cell receptor CD22 antibody
    • B-lymphocyte cell adhesion molecule antibody
    • BL CAM antibody
    • BL-CAM antibody
    • BLCAM antibody
    • CD 22 antibody
    • CD22 antibody
    • CD22 antigen antibody
    • CD22 molecule antibody
    • CD22 protein antibody
    • CD22_HUMAN antibody
    • Lectin 2 antibody
    • Leu14 antibody
    • Lyb8 antibody
    • MGC130020 antibody
    • sialic acid binding Ig like lectin 2 antibody
    • Sialic acid binding immunoglobulin like lectin 2 antibody
    • Sialic acid-binding Ig-like lectin 2 antibody
    • SIGLEC 2 antibody
    • Siglec-2 antibody
    • SIGLEC2 antibody
    • T cell surface antigen Leu 14 antibody
    • T-cell surface antigen Leu-14 antibody
    see all

图片

  • Overlay histogram showing peripheral blood lymphocytes stained with ab1172 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1172, 0.03 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

文献

ab1172 has not yet been referenced specifically in any publications.

客户评价及客户问答

Question

Product Information Product Name: CD22 antibody Cat. # ab1172 Lot No: Ordered:June 2011 PO Number: 061511AC 1. Order details: • Antibody storage conditions (temperature/reconstitution etc) 4℃ 2. Please describe the problem (high background, low signal, no signal etc). No signal 3. On what material are you testing the antibody in FACS: • Species: Human • Cell type: EBV immortalized B cell • Other details: 4. The cells • Which buffer did you use for cell suspension (PBS) • How many cells did you use 105 5. Did you permeabilize the cells? NO • Permeabilising agent: saponin, triton, other. NO • concentration: diluted by 1:50,1:100 and 1:200 • Incubation time • Incubation temperature: 6. Primary antibody Specification (in which species was it raised against): • At what dilution(s) have you tested this antibody: diluted by 1:50,1:100 and 1:200 • What dilution buffer was used:PBS+1%BSA • Incubation time:30min • Incubation temperature: at room temperature, about 26℃ • • What washing steps were done: PBS+2%FBS washed twice 7. Secondary antibody Specification (in which species was it raised against): • At what dilution(s) have you tested this antibody: • What dilution buffer was used: • Incubation time: • Incubation temperature:: • What washing steps were done: 8. Which detection system did you use • System: BD FCScalibur • Detection wavelength:FITC 9. Did you apply positive and negative controls along with the samples. Please specify. We also use the ab23620 to stain CD22 of the EBV immortalized B cell line, the signal is good, but the ab1172 could not detect any signal. 10. Optimization attempts • How many times have you tried the FACS? Twice • Do you obtain the same results every time? Yes • What steps have you altered? At higher concentration

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab1172. I would also appreciate if you can confirm some further details: 1. Please confirm which particular cell line has been used? Please provide more details. What expression level of CD22 is expected? 2. Could you confirm if a postive control such as fresh lymphocytes or Raji cell been used? I can suggest this would be beneficial. 3. To increase the signal, I can recommend to try a higher dilution of 1:10 (10 µl per 1x10e6 cells in 100 µl sample as suggested on the datasheet). In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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