Anti-CD19抗体[CB19] (Phycoerythrin) (ab1168)

概述

  • 产品名称
    Anti-CD19抗体[CB19] (Phycoerythrin)
    参阅全部 CD19 一抗
  • 描述
    小鼠单克隆抗体[CB19] to CD19 (Phycoerythrin)
  • 宿主
    Mouse
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human, Chimpanzee, Macaque monkey, Rhesus monkey

性能

应用

Our Abpromise guarantee covers the use of ab1168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient. CD19 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers. 10µl of CD19 (PE) is sufficient for labelling of 1x106 cells.

 

 

 

 

ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能
    Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
  • 疾病相关
    Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 序列相似性
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • 翻译后修饰
    Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antibody deficiency due to defect in CD19, included antibody
    • AW495831 antibody
    • B lymphocyte antigen CD19 antibody
    • B lymphocyte surface antigen B4 antibody
    • B-lymphocyte antigen CD19 antibody
    • B-lymphocyte surface antigen B4 antibody
    • B4 antibody
    • CD19 antibody
    • CD19 antigen antibody
    • CD19 molecule antibody
    • Cd19 protein antibody
    • CD19_HUMAN antibody
    • CVID3 antibody
    • Differentiation antigen CD19 antibody
    • Leu 12 antibody
    • Leu-12 antibody
    • Leu12 antibody
    • MGC109570 antibody
    • MGC12802 antibody
    • T-cell surface antigen Leu-12 antibody
    see all

图片

  • ab1168 used in Flow Cytometry.
    Whole PBMC cells from Rhesus monkey were fixed with paraformaldehyde. The lymphocyte population was gated based on forward and side scatter properties. The cells within the gate were then analysis on 2 color dot plots with the CD19 (clone CB19) plotted on one axis and other lineage makers plotted on the other axis. CD3 and NKG2a positive population were negative for CD19 staining.All CD20 positive cells also stained positive for CD19. Addition analysis was carried out to verify that the monocyte and granulocyte populations were negative for staining with this CD19 antibody as is expected.

    ab1168 used undiluted.

    See Abreview

文献

This product has been referenced in:
  • Pyöriä L  et al. Extinct type of human parvovirus B19 persists in tonsillar B cells. Nat Commun 8:14930 (2017). Flow Cyt . Read more (PubMed: 28374737) »
  • Bradbury LE  et al. The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules. J Immunol 149:2841-50 (1992). Read more (PubMed: 1383329) »
See all 3 Publications for this product

客户评价及客户问答

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that the homology between human and Macaca fascicularis (Crab-eating macaque,Cynomolgus monkey) CD19 is 83%. (alignment of the full length sequences:P15391 andG7Q0T7)

This is border line, but I would still be happy to offer your customer the testing discount as mentioned in the previous email.

Please let me know if you would like me to issue the testing discount code for your customer. To issue this I will need the customer's email address.

I am looking forward to your reply.

Read More

Answer

Thank you for your inquiry.

I can confirm that the immunogen used to obtain clone CB19 is a human chronic lymphocytic leukemia cell line.

Wetherefore do nothave the specific immunogen sequence used to obtain this clone and to our knowledge it was not further mapped.

Could you please let me know which non human primate your customer uses? If the homology of CD19 is 85% or higher and this non human primate has not been tested yet, your customer might be eligible for your testing discount program.

For example, the homology of total CD19 with Callithrix jacchus is 88% and therefore it would be worth trying this antibody with these samples.

I am sorry I did not have a definite answer for your customer on this occasion and hope this information is nevertheless helpful.

Read More

Answer

Thank you for contacting us.

Unfortunately, we don’t have any data to support the cross reactivity of this antibody with Cynomolgus monkey.

However, if you are interested in testing ab1168 in Cynomolgus monkey with Flow Cytometry, I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

Therefore, I can offer a discount off a future purchase if you buy ab1168 and test it in Cynomolgus monkey and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab1168 with Cynomolgus monkey samples. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in Flow Cytometry with Cynomolgus monkey.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rhesus monkey Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Whole blood staining and PBMC isolated on a ficoll spin.
Sample buffer: PBS with 2% FCS
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
The lymphocyte population was gated based on forward and side scatter properties. The cells within the gate were then analysis on 2 color dot plots with the CD19 (clone CB19) plotted on one axis and other lineage makers plotted on the other axis. CD3 and NKG2a positive population were negative for CD19 staining.All CD20 positive cells also stained positive for CD19. Addition analysis was carried out to verify that the monocyte and granulocyte populations were negative for staining with this CD19 antibody as is expected.

Ken Rogers

Verified customer

提交于 Oct 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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