Anti-CD15抗体[MEM-158] (ab665)
Key features and details
- Mouse monoclonal [MEM-158] to CD15
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-CD15抗体[MEM-158]
参阅全部 CD15 一抗 -
描述
小鼠单克隆抗体[MEM-158] to CD15 -
宿主
Mouse -
特异性
Human CD15 antigen. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue/ cell preparation (Human granulocytes).
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阳性对照
- Flow Cyt: Human peripheral whole blood.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.00
Preservative: 0.097% Sodium azide
Constituent: Tris buffered saline -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-158 -
同种型
IgM -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use a concentration of 1 - 4 µg/ml. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
靶标
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相关性
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
细胞定位
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
别名
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
图片
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Flow Cytometry analysis of human peripheral whole blood labeling CD15 (surface staining) with ab665 at 0.3 µg/mL. CD15 positive human neutrophil granulocytes (red-filled). CD15 negative lymphocytes (black-dashed).
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Flow cytometry surface staining pattern of human peripheral blood stained using Anti-CD15 antibody [MEM-158] (ab665) at 0.3 µg/mL.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab665 被引用在 1 文献中.
- Kishimoto T. (Eds.) et al. Leucocyte typing VI. Garland Publishing Inc. : (1997). PubMed: