Anti-CD147抗体(ab232967)
Key features and details
- Rabbit polyclonal to CD147
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-CD147抗体
参阅全部 CD147 一抗 -
描述
兔多克隆抗体to CD147 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (His-T7-tag) corresponding to Human CD147 aa 50-300. Two N-terminal tags, His-tag and GST-tag. Expressed in E.coli.
Database link: P35613-1 -
阳性对照
- IHC-P: Human prostate cancer, kidney, glioma, stomach, stomach cancer, colorectal cancer tissue. ICC/IF: HeLa cells. WB: Recombinant human CD147 protein; HeLa cell lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Protein A purified -
纯化说明
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232967于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
|
WB |
Use a concentration of 0.5 - 2 µg/ml.
|
|
ICC/IF |
Use a concentration of 5 - 20 µg/ml.
|
说明 |
---|
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. |
ICC/IF
Use a concentration of 5 - 20 µg/ml. |
靶标
-
功能
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
组织特异性
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
数据库链接
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
-
别名
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
图片
-
Anti-CD147 antibody (ab232967) at 2 µg/ml + Recombinant human CD147 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution -
Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling CD147 using ab232967 at 20 µg/ml in ICC/IF. FITC staining.
-
Anti-CD147 antibody (ab232967) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution -
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human stomach tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human glioma tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human kidney tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
-
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab232967 被引用在 1 文献中.
- De Caluwé L et al. The CD147 Protein Complex Is Involved in Entry of Chikungunya Virus and Related Alphaviruses in Human Cells. Front Microbiol 12:615165 (2021). PubMed: 33717005