Anti-CD14抗体[4B4F12] (ab182032)
Key features and details
- Mouse monoclonal [4B4F12] to CD14
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
-
产品名称
Anti-CD14抗体[4B4F12]
参阅全部 CD14 一抗 -
描述
小鼠单克隆抗体[4B4F12] to CD14 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human CD14 aa 1-250. Expressed in E. coli.
Database link: P08571 -
阳性对照
- Human CD14 recombinant protein (amino acids 20-214), lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc, HepG2, A549, HL60, mouse RAW264.7, Hela, HEK293 and mouse NIH 3T3 cell lysates. Jurkat cells. Human stomach cancer tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
4B4F12 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
|
|
IHC-P | (1) |
1/200 - 1/1000.
|
Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
组织特异性
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
序列相似性
Contains 11 LRR (leucine-rich) repeats. -
翻译后修饰
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 929 Human
- Entrez Gene: 12475 Mouse
- Omim: 158120 Human
- SwissProt: P08571 Human
- SwissProt: P10810 Mouse
- Unigene: 163867 Human
-
别名
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
图片
-
Flow cytometric analysis of Jurkat cells labeling CD14 using ab182032 at 1/200 (green) and a negative control (red).
-
Immunohistochemical analysis of paraffin-embedded, DAB-stained Human stomach cancer tissues labeling CD14 using ab182032 at 1/200.
-
Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution + Human CD14 recombinant protein (amino acids 20-214)
Predicted band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue section labeling CD14 with ab182032. The tissue was fixed with formaldehyde, heat mediated antigen retrieval was performed followed by blocking with 5% serum for 12 hours at 4°C. The tissue was incubated with undiluted Anti-CD14 antibody [4B4F12] (ab182032) for 12 hours at 4°C.
-
All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution
Lane 1 : Lysate from HEK293 cells
Lane 2 : Lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc
Predicted band size: 40 kDa -
All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : mouse RAW264.7 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : HEK293 cell lysate
Lane 7 : mouse NIH 3T3 cell lysate
Predicted band size: 40 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (28)
ab182032 被引用在 28 文献中.
- Guo D et al. ELK1-Induced upregulation of long non-coding TNK2-AS1 promotes the progression of acute myeloid leukemia by EZH2-mediated epigenetic silencing of CELF2. Cell Cycle 22:117-130 (2023). PubMed: 35941836
- Mo YQ et al. Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome. J Clin Invest 132:N/A (2022). PubMed: 35113815
- Nakamura A et al. The potential of soluble CD14 in discriminating nonalcoholic steatohepatitis from nonalcoholic fatty liver disease. Hepatol Res 52:508-521 (2022). PubMed: 35129841
- Wu Z et al. sGRP78 enhances selective autophagy of monomeric TLR4 to regulate myeloid cell death. Cell Death Dis 13:587 (2022). PubMed: 35798718
- Zadka Ł et al. Interplay of stromal tumor-infiltrating lymphocytes, normal colonic mucosa, cancer-associated fibroblasts, clinicopathological data and the immunoregulatory molecules of patients diagnosed with colorectal cancer. Cancer Immunol Immunother 70:2681-2700 (2021). PubMed: 33625532