重组Anti-CD133抗体[EPR24348-38] (ab271092)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24348-38] to CD133
- Suitable for: Flow Cyt (Intra), IHC-Fr, IP, WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-CD133抗体[EPR24348-38]
参阅全部 CD133 一抗 -
描述
兔单克隆抗体[EPR24348-38] to CD133 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-Fr, IP, WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse kidney, Mouse E14.5 brain, Mouse kidney, Mouse kidney tissue treated with PNGase F lysates. IHC-P: Mouse liver, Mouse pancreas, Mouse retina, Mouse kidney tissues. IHC-Fr: Mouse E14.5 embryonic cortex, Mouse E14.5 embryonic kidney, Mouse E14.5 embryonic lung tissues. IP: Mouse E14.5 brain tissue lysate. Flow Cyt-intra: Mouse bone marrow cell
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR24348-38 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/50.
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IHC-Fr |
1/100.
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IP |
1/30.
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WB |
1/1000. Predicted molecular weight: 97 kDa.
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IHC-P |
1/2000.
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说明 |
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Flow Cyt (Intra)
1/50. |
IHC-Fr
1/100. |
IP
1/30. |
WB
1/1000. Predicted molecular weight: 97 kDa. |
IHC-P
1/2000. |
靶标
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功能
May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). -
组织特异性
Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). -
疾病相关
Retinitis pigmentosa 41
Cone-rod dystrophy 12
Stargardt disease 4
Retinal macular dystrophy 2 -
序列相似性
Belongs to the prominin family. -
翻译后修饰
Isoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. -
细胞定位
Apical cell membrane. Cell projection, microvillus membrane. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. - Information by UniProt
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数据库链接
- Entrez Gene: 19126 Mouse
- SwissProt: O54990 Mouse
- Unigene: 6250 Mouse
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别名
- AC133 antibody
- Antigen AC133 antibody
- CD133 antibody
see all
图片
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Flow Cytometry analysis of Mouse bone marrow cells labelling CD133 with ab271092 at 1/50 (right). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as the isotype control (left). Gated on viable cells.
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Flow Cytometry analysis of Mouse bone marrow cells labelling CD133 with ab271092 at 1/50 (right). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG (Alexa Fluor® 647) (ab150083) at 1/2000 was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as the isotype control (left). Cells were stained with rabbit IgG or ab271092. Then stained with Lineage cocktail conjugated to FITC, anti-Sca-1 conjugated to PE/Cy7, anti-CD117 conjugated to APC/Cy7 and anti-CD16/32 conjugated to BV510.
Gated on viable MP cells. Positive staining on CD16/32+ MP cells is consistent with literature. (PMID: 23509298). -
Flow Cytometry analysis of Mouse bone marrow cells labelling CD133 with ab271092 at 1/50 (right). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) was used as the isotype control (left). Cells were co-stained with anti-CD117 conjugated to BV421. Gated on viable cells.
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All lanes : Anti-CD133 antibody [EPR24348-38] (ab271092) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse E14.5 brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: mouse heart (PMID:17575139).
Exposure time: Lanes 1-2: 10 seconds; Lane 3: 70 seconds.
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All lanes : Anti-CD133 antibody [EPR24348-38] (ab271092) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse kidney tissue lysate treated with PNGase F
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 97 kDa
Observed band size: 120,97 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the biliary ducts of mouse liver. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800(Bond™ Polymer Refine Detection). Positive staining on mouse pancreatic ducts. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the cone and rod cells of mouse retina. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling CD133 with ab271092 at 1/2000 (0.23 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with ab271092 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic cortex tissue labeling CD133 with ab271092 at 1/100 (4.59 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on the apical membrane of mouse E14.5 embryonic cortex is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic kidney tissue labeling CD133 with ab271092 at 1/100 (4.59 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on the apical membrane of mouse E14.5 embryonic kidney is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic lung tissue labeling CD133 with ab271092 at 1/100 (4.59 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on the apical membrane of lung buds is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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CD133 was immunoprecipitated from 0.35 mg Mouse E14.5 brain tissue lysate 10 ug with ab271092 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271092 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse E14.5 brain tissue lysate 10 ug
Lane 2: ab271092 IP in Mouse E14.5 brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271092 in Mouse E14.5 brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab271092 被引用在 2 文献中.
- Yang H et al. Knockdown of zinc finger protein 267 suppresses diffuse large B-cell lymphoma progression, metastasis, and cancer stem cell properties. Bioengineered 13:1686-1701 (2022). PubMed: 35001816
- Cigliano A et al. The Hippo pathway effector TAZ induces intrahepatic cholangiocarcinoma in mice and is ubiquitously activated in the human disease. J Exp Clin Cancer Res 41:192 (2022). PubMed: 35655220