Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (INS-1)
Permeabilization
Yes - 0.5% TritonX100
Specification
INS-1
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/100
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
Nuclear staining, not expected
Abcam response
The reviewer has used this antibody in a tested species and application. We have offered a refund or replacement as per our Abpromise guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 02 2020