重组Anti-CD11b抗体[SP330] - C-terminal (ab224805)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP330] to CD11b - C-terminal
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD11b抗体[SP330] - C-terminal
参阅全部 CD11b 一抗 -
描述
兔单克隆抗体[SP330] to CD11b - C-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: TF-1 cell lysate. IHC-P: Human tonsil, colon, spleen, bone marrow, lung, cerebellum, thymus, lymph node, HK lymphoma, pancreatic adenocarcinoma and endometrial adenocarcinoma tissues. Flow Cyt: TF-1 cells.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP330 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224805于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/100.
Primary incubation for 30 minutes at at 4°C. |
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WB |
1/400. Predicted molecular weight: 127 kDa.
Primary incubation for 1 hour at room temperature. |
|
IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 10 minutes at room temperature. |
说明 |
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Flow Cyt
1/100. Primary incubation for 30 minutes at at 4°C. |
WB
1/400. Predicted molecular weight: 127 kDa. Primary incubation for 1 hour at room temperature. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 10 minutes at room temperature. |
靶标
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功能
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
组织特异性
Predominantly expressed in monocytes and granulocytes. -
疾病相关
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
序列相似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
结构域
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3684 Human
- Omim: 120980 Human
- SwissProt: P11215 Human
- Unigene: 172631 Human
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别名
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
图片
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Flow cytometric analysis of TF-1 (human bone marrow erythroleukemia cell line) cell line labeling CD11b with ab224805 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue) .
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Anti-CD11b antibody [SP330] - C-terminal (ab224805) at 1/100 dilution + TF-1 (human bone marrow erythroleukemia cell line) cell lysate
Predicted band size: 127 kDa -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human spleen tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cerebellum tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224805 被引用在 1 文献中.
- Masugi Y et al. Characterization of spatial distribution of tumor-infiltrating CD8+ T cells refines their prognostic utility for pancreatic cancer survival. Mod Pathol 32:1495-1507 (2019). PubMed: 31186528