Anti-CD11b + CD11c抗体[OX42] (ab1211)
![Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)](https://www.abcam.cn/ps/products/1/ab1211/Images/ab1211-273171-anti-cd11b-c-antibody-ox42-immunofluorescence.jpg "Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)")
Key features and details
- Mouse monoclonal [OX42] to CD11b + CD11c
- Suitable for: IHC-Fr, ICC
- Reacts with: Rat
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-CD11b + CD11c抗体[OX42] -
描述
小鼠单克隆抗体[OX42] to CD11b + CD11c -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, ICCmore details
不适用于: IHC-P -
种属反应性
与反应: Rat
不与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to CD11b. Resident peritoneal macrophages from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat.
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阳性对照
- ICC: NR8383 cells. IHC-Fr: Rat spleen and brain tissue.
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常规说明
IHC protocol advice:
This antibody is not suitable for IHC on paraffin-embedded samples.
For best results in IHC on frozen tissue, the following may help detection:
1) Paraformaldehyde perfusion fixed samples have worked well for many customers.
2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
3) PFA-fixed samples will require cryoprotection by sucrose infiltration.
4) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.
5) Do not heat the samples or sections.
6) During the staining procedure, do not allow the sections to dry out.Our Technical team (technical@abcam.com) will be happy to provide further information and advice.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading... -
纯度
Protein G purified -
Primary antibody说明
The principal use of this antibody is in the study of functional heterogeneity of macrophages; it may be used to follow macrophage differentiation and to investigate the function of the CD11b + CD11c equivalent antigen. -
克隆
单克隆 -
克隆编号
OX42 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| ICC |
Use a concentration of 1 - 5 µg/ml.
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| 说明 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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ICC
Use a concentration of 1 - 5 µg/ml. |
靶标
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细胞定位
CD11b: Membrane. CD11c: Membrane. -
数据库链接
图片
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Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)Image courtesy of Carl Hobbs.
ab1211 staining CD11b + CD11c (shown in red) in rat spleen tissue sections by IHC (PFA perfusion fixed frozen sections). Tissue samples were fixed with formaldehyde and blocked with BSA for 10 minutes at 21°C. The sample was incubated with primary antibody (1/2000 in TBS/BSA/azide) at 21°C for 16 hours. A Biotin-conjugated Goat anti mouse polyclonal (1/300) was used as the secondary antibody.
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![Immunocytochemistry - Anti-CD11b + CD11c antibody [OX42] (ab1211)](https://www.abcam.cn/ps/products/1/ab1211/Images/ab1211-10-anti-cd11b-cd11c-ox42-antibody-immunocytochemistry-nr8383-rat.jpg)
Immunocytochemistry - Anti-CD11b + CD11c antibody [OX42] (ab1211)ab1211 staining CD11b + CD11c in NR8383 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab1211 at 1 µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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![Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)](https://www.abcam.cn/ps/products/1/ab1211/Images/ab1211-4083-anti-cd11b-c-antibody-ox42-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] (ab1211)Image courtesy of an anonymous Abreview.ab1211 staining CD11b + CD11c (shown in green) in rat spleen tissue by IHC (Frozen sections). Tissue was fixed in formaldehyde, blocked with 10% serum for 30 minutes at 24°C, then incubated with ab1211 at a 1/100 dilution. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal, used at a 1/1000 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (103)
ab1211 被引用在 103 文献中.
- Ding H et al. BDNF promotes activation of astrocytes and microglia contributing to neuroinflammation and mechanical allodynia in cyclophosphamide-induced cystitis. J Neuroinflammation 17:19 (2020). PubMed: 31931832
- Wang Y et al. Electroacupuncture Treatment Suppresses Transcription Factor IRF8 in Spinal Cord of Rats with Spared Nerve Injury. Pain Res Manag 2020:1854363 (2020). PubMed: 32351637
- Hu L et al. Microglia-Derived NLRP3 Activation Mediates the Pressor Effect of Prorenin in the Rostral Ventrolateral Medulla of Stress-Induced Hypertensive Rats. Neurosci Bull 36:475-492 (2020). PubMed: 32242284
- He D et al. Asthmatic Airway Vagal Hypertonia Involves Chloride Dyshomeostasis of Preganglionic Neurons in Rats. Front Neurosci 14:31 (2020). PubMed: 32082109
- Kan LK et al. P2X7 receptor antagonism inhibits tumour growth in human high-grade gliomas. Purinergic Signal 16:327-336 (2020). PubMed: 32583309