概述

  • 产品名称
    Anti-CD10抗体[56C6]
    参阅全部 CD10 一抗
  • 描述
    小鼠单克隆抗体[56C6] to CD10
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Cat, Dog, Human
    预测可用于: Mouse
  • 免疫原

    Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein

  • 阳性对照
    • IHC-P: MDA-MB-231 human breast cancer cells grown in mouse. Renal cell carcinoma tissue.
  • 常规说明

    We have mixed customer feedback about the mouse specificity, which is why we no longer guarantee this species. Contact scientific support team for any inquiry.

    This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.

性能

应用

Our Abpromise guarantee covers the use of ab951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 85 kDa. PubMed: 26074811
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-).

靶标

  • 功能
    Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
  • 序列相似性
    Belongs to the peptidase M13 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Atriopeptidase antibody
      • CALLA antibody
      • CD10 antibody
      • CD10 antigen antibody
      • Common acute lymphocytic leukemia antigen antibody
      • DKFZp686O16152 antibody
      • EC 3.4.24.11 antibody
      • Enkephalinase antibody
      • EPN antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
      • Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
      • Membrane metallo endopeptidase antibody
      • Membrane metallo endopeptidase variant 1 antibody
      • Membrane metallo endopeptidase variant 2 antibody
      • Membrane metalloendopeptidase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
      • Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
      • Membrane metalloendopeptidase variant 1 antibody
      • Membrane metalloendopeptidase variant 2 antibody
      • MGC126681 antibody
      • MGC126707 antibody
      • MME antibody
      • NEP antibody
      • NEP_HUMAN antibody
      • Neprilysin antibody
      • neprilysin-390 antibody
      • neprilysin-411 antibody
      • Neutral endopeptidase 24.11 antibody
      • Neutral endopeptidase antibody
      • Neutral endopeptidase, membrane-associated antibody
      • SFE antibody
      • Skin fibroblast elastase antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)

      Paraffin embedded renal cell carcinoma tissue stained for CD10 with ab951 (1/25 dilution) in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)This image is courtesy of an anonymous Abreview.

      ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.

      See Abreview

    文献

    This product has been referenced in:
    • Li X  et al. Encapsulated papillary carcinoma of the breast: A clinicopathological study of 49 cases. Curr Probl Cancer N/A:N/A (2018). Read more (PubMed: 29731165) »
    • Zhu L  et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »

    See all 26 Publications for this product

    发表研究结果有使用 ab951？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    15 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Frozen sections) abreview for Anti-CD10 antibody [56C6]

     Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Uterus)
    Permeabilization
    Yes - 0.5% Triton/PBS
    Specification
    Uterus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Jul 29 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD10 antibody [56C6]

     Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (DLCBL cell line WSUDLCL2 xenograft)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
    Permeabilization
    No
    Specification
    DLCBL cell line WSUDLCL2 xenograft
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Dec 16 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD10 antibody [56C6]

     Good
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mammary gland)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM pH=6.0
    Permeabilization
    Yes - PBS with 3% Triton
    Specification
    mammary gland
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    提交于 Aug 04 2015

    We have purchased Mouse monoclonal [56C6] to CD10 ab recently and could you please provide the concentration?


    The IgG concentration of ab951 is 7.2µg/mL.

    the ordered was placed on xxxx, the PO number is xxx. We would like another Ab951 sample (but of different lot , last lot is  xxxx. Below is the shipping address:

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

    Read More

    I followed exactly the protocol in the paper by Devi L et al you cited, unfortunately, I still did not see any ~100kd band ( see the attached jpg, the whole brain homogenates sample lane),  I also adopted another protocol to fractionate the mouse brain...

    Read More

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    I am sorry that these protocol details did not yield the expected results. The details p...

    Read More

    Inquiry: Dear Madam and Sir, We bought Mouse anti-CD10 (56C6) N:ab951, lot Number: GR38260-2. I am wondering if you could tell me the Ab concentration of this product exactly. Thank you very much and best regards

    Thank you for contacting us and your interest in our products.

    The concentration of the Anti-CD10 antibody [56C6] (ab951), lot number GR38260-2 is 7.2µg/mL.

    I hope this information has been of help. If you have any further que...

    Read More

    We have purchased two tubes of mouse CD10 antibody (56C6, catalogue ab951). Both of them give me expected band right below 100KD for NB7 cell lysate ( human neuroblastoma cell line known to have high CD10 expression) and 293T cells transfected with pLV...

    Read More

    Thank you for contacting us.

    I am sorry that this product has not been performing as it should in mouse.This product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product i...

    Read More

    Product code: 951
    Lot number: GR79429-1
    Inquiry: How should the Renoir Red solution be used in conjunction with the CD10 antibody? Also, no concentration was listed on the tube or the product sheet, although it was specified that the antibody...

    Read More

    Thank you for contacting us.

    The IgG concentration for lot GR79429-1 is 7.2µg/mL. For IHC, the antibody should be used at a concentration of 1/20 - 1/50. The antibody was validated for IHC with a sensitive polymer detection system, so if you...

    Read More

    LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Multiple bands. The expected molecular weight is 85 kDa, whereas I got a major slower band at about 100 kDa (which I believe is the correct band) and a faster, m...

    Read More

    Thank you for contacting Abcam.

    I think you are correct that the band that you are seeing at 100kDa is correct as based on the Swissprot sequence http://www.uniprot.org/uniprot/Q61391, there are q few glycosylation sites on this protein that ...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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