重组Anti-CCR9抗体[4G2] (ab288375)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [4G2] to CCR9
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Human
概述
-
产品名称
Anti-CCR9抗体[4G2]
参阅全部 CCR9 一抗 -
描述
兔单克隆抗体[4G2] to CCR9 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human CCR9.
Database link: P51686 -
阳性对照
- WB: SH-SY5Y, HepG2, Jurkat, HL-60, Hela, U87 whole cell lysate, Mouse brain tissue ICC/IF: HepG2 cells. IP: HL-60 whole cell lysate
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
-
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
4G2 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab288375于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/5000. Predicted molecular weight: 42 kDa.
|
|
ICC/IF |
1/20 - 1/200.
|
|
IP |
1/200 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/5000. Predicted molecular weight: 42 kDa. |
ICC/IF
1/20 - 1/200. |
IP
1/200 - 1/1000. |
靶标
-
功能
Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
组织特异性
Highly expressed in the thymus and low in lymph nodes and spleen. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 10803 Human
- Entrez Gene: 12769 Mouse
- Omim: 604738 Human
- SwissProt: P51686 Human
- SwissProt: Q9WUT7 Mouse
- Unigene: 225946 Human
- Unigene: 440604 Mouse
- Unigene: 442383 Mouse
-
别名
- C C chemokine receptor D6 antibody
- C C chemokine receptor type 9 antibody
- C C CKR 9 antibody
see all
图片
-
All lanes : Anti-CCR9 antibody [4G2] (ab288375) at 1 µg/ml
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HL-60 whole cell lysate
Lane 5 : Hela whole cell lysate
Lane 6 : U87 whole cell lysate
Lane 7 : Mouse brain tissue
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
Immunofluorescence staining of HepG2 cells with ab288375 at 1/33 dilution, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
-
Immunoprecipitating CCR9 in HL-60 whole cell lysate
Lane 1: Rabbit control IgG instead of ab288375 in HL-60 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ab288375 (3μg) + HL-60 whole cell lysate (500μg)
Lane 3: HL-60 whole cell lysate (20μg)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab288375 尚未被引用在任何文献中。