重组Anti-CCR8抗体[E77] - BSA and Azide free (ab239814)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E77] to CCR8 - BSA and Azide free
- Suitable for: ELISA, IP, WB
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
概述
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产品名称
Anti-CCR8抗体[E77] - BSA and Azide free
参阅全部 CCR8 一抗 -
描述
兔单克隆抗体[E77] to CCR8 - BSA and Azide free -
宿主
Rabbit -
特异性
Does not cross-react with other G-protein coupled receptor 1 family members.
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经测试应用
适用于: ELISA, IP, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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表位
Residues in loop 2 extracellular domain of human CCR8 -
常规说明
ab239814 is the carrier-free version of ab32399.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E77 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab239814于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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说明 |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
靶标
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功能
Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1237 Human
- Omim: 601834 Human
- SwissProt: P51685 Human
- Unigene: 113222 Human
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别名
- C C chemokine receptor type 8 antibody
- C C CKR 8 antibody
- C C motif chemokine receptor 8 antibody
see all
图片
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Anti-CCR8 antibody [E77] (ab32399) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaThis data was developed using ab32399, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab32399, the same antibody clone in a different buffer formulation.
ab32399 (purified) at 1/110 dilution (20 µg/mL) immunoprecipitating CCR8 in Human thymus lysate. Human thymus lysate 10 µgab32399 & Human thymus lysateRabbit monoclonal IgG (ab172730) instead of ab32399 in Human thymus lysate
For western blotting, ab32399 at 1/500 dilution (4.45 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
This data was developed using ab32399, the same antibody clone in a different buffer formulation.
ELISA using Human CCR8 Antigen at a concentration of 1000 ng/ml; and purified ab32399 at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at a 1:2500 dilution. -
Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human Thymus lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaThis data was developed using ab32399, the same antibody clone in a different buffer formulation.
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Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human spleen tissue lysate at 15 µg
Secondary
Goat anti-Rabbit IgG, (H+L), peroxidase conjugated at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaThis data was developed using ab32399, the same antibody clone in a different buffer formulation.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
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