重组Anti-CCR8抗体[E77] (ab32399)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E77] to CCR8
- Suitable for: ELISA, WB, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
概述
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产品名称
Anti-CCR8抗体[E77]
参阅全部 CCR8 一抗 -
描述
兔单克隆抗体[E77] to CCR8 -
宿主
Rabbit -
特异性
Does not cross-react with other G-protein coupled receptor 1 family members. -
经测试应用
适用于: ELISA, WB, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human CCR8.
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表位
Residues in loop 2 extracellular domain of human CCR8 -
阳性对照
- WB: Human Thymus and spleen tissue lysates; A431 cell lysate IP: Human thymus tissue lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E77 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32399于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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IP |
1/110.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000 - 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IP
1/110. |
靶标
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功能
Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1237 Human
- Omim: 601834 Human
- SwissProt: P51685 Human
- Unigene: 113222 Human
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别名
- C C chemokine receptor type 8 antibody
- C C CKR 8 antibody
- C C motif chemokine receptor 8 antibody
see all
图片
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Anti-CCR8 antibody [E77] (ab32399) at 1/1000 dilution (Purified) + A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaBlocking/Diluting Buffer and concentration: 5% NFDM/TBST
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ab32399 (purified) at 1/110 dilution (20 µg/mL) immunoprecipitating CCR8 in Human thymus lysate. Human thymus lysate 10 µgab32399 & Human thymus lysateRabbit monoclonal IgG (ab172730) instead of ab32399 in Human thymus lysate
For western blotting, ab32399 at 1/500 dilution (4.45 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST . -
ELISA using Human CCR8 Antigen at a concentration of 1000 ng/ml; and purified ab32399 at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at a 1:2500 dilution.
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Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human Thymus lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
Anti-CCR8 antibody [E77] (ab32399) at 1/5000 dilution (unpurified) + Human spleen tissue lysate at 15 µg
Secondary
Goat anti-Rabbit IgG, (H+L), peroxidase conjugated at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab32399 被引用在 1 文献中.
- Liu Y & Zhang Y Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction. Aging (Albany NY) 13:20383-20394 (2021). PubMed: 34413269