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Anti-CCR5抗体(ab7346)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)
  • Western blot - Anti-CCR5 antibody (ab7346)
  • Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)

Key features and details

  • Rabbit polyclonal to CCR5
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-CCR5抗体
    参阅全部 CCR5 一抗
  • 描述

    兔多克隆抗体to CCR5
  • 宿主

    Rabbit
  • 特异性

    This antibody recognises the N-terminal region of the chemokine receptor CCR5.
  • 经测试应用

    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).
    Sequence:

    SSPIYDINYYTSEPC


    (Peptide available as ab7876)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • THP-1 whole cell lysate
  • 常规说明

    HIV and chemokine receptor

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Signaling Pathway
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    • G protein signaling
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    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • THP-1 whole cell lysate (ab7913)
  • Recombinant Protein

    • Recombinant Human CCR5 protein (ab158144)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7346于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 20 µg/ml.
WB
1/1000 - 1/2000. Detects a band of approximately 40 kDa.
ICC/IF
Use at an assay dependent concentration.
说明
IHC-P
Use a concentration of 20 µg/ml.
WB
1/1000 - 1/2000. Detects a band of approximately 40 kDa.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
  • 组织特异性

    Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
  • 疾病相关

    Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰

    Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
    O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
    Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
    Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P51681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1234 Human
    • Omim: 601373 Human
    • SwissProt: P51681 Human
    • Unigene: 450802 Human
    • 别名

      • AM4 7 antibody
      • C C chemokine receptor type 5 antibody
      • C C CKR 5 antibody
      • C-C chemokine receptor type 5 antibody
      • C-C CKR-5 antibody
      • C-C motif chemokine receptor 5 A159A antibody
      • CC Chemokine Receptor 5 antibody
      • CC Chemokine Receptor Type 5 antibody
      • CC CKR 5 antibody
      • CC-CKR-5 antibody
      • CCCKR 5 antibody
      • CCCKR5 antibody
      • CCR 5 antibody
      • CCR-5 antibody
      • CCR5 antibody
      • CCR5 chemokine (C C motif) receptor 5 antibody
      • CCR5_HUMAN antibody
      • CD 195 antibody
      • CD195 antibody
      • CD195 Antigen antibody
      • Chemokine C C motif receptor 5 antibody
      • Chemokine receptor CCR5 antibody
      • CHEMR13 antibody
      • CKR 5 antibody
      • CKR5 antibody
      • CMKBR 5 antibody
      • CMKBR5 antibody
      • FLJ78003 antibody
      • HIV 1 Fusion Coreceptor antibody
      • HIV-1 fusion coreceptor antibody
      • HIV1 fusion coreceptor antibody
      • IDDM22 antibody
      • MIP-1 alpha receptor antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)

      Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.

    • Western blot - Anti-CCR5 antibody (ab7346)
      Western blot - Anti-CCR5 antibody (ab7346)
      Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate

      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)
      Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody (ab7346)

      Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)

      ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab7346?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab7346 被引用在 5 文献中.

    • Mikhailova A  et al. Antiapoptotic Clone 11-Derived Peptides Induce In Vitro Death of CD4+ T Cells Susceptible to HIV-1 Infection. J Virol 94:N/A (2020). PubMed: 32350074
    • Kuzmina A  et al. Functional Mimetics of the HIV-1 CCR5 Co-Receptor Displayed on the Surface of Magnetic Liposomes. PLoS One 10:e0144043 (2015). PubMed: 26629902
    • Jiang AP  et al. Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells. J Virol 90:2928-37 (2015). PubMed: 26719250
    • Levina V  et al. Role of eotaxin-1 signaling in ovarian cancer. Clin Cancer Res 15:2647-56 (2009). IHC-P ; Human . PubMed: 19351767
    • Dittmer A  et al. Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10. Cell Mol Life Sci 66:3053-65 (2009). WB ; Human . PubMed: 19603142

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-8 of 8 Abreviews or Q&A

    Question

    Good day   I am a postdoctoral scientist at the NIH and I am interested in purchasing one of your CCR5 peptides. However I am not sure which of the peptide products (ab92719, ab95861 or ab7876) might be more suited to my experiment.   I am working on anti-HIV targets and I want to test (via ELISAs) if my target inhibits the gp120-CCR5 binding. Could you recommend one of your CCR5 peptides (and corresponding anti-CCR5 antibody) that might be most suited for my application?   Thanks and regards

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2012

    Answer

    Thank you for contacting Abcam.



    These peptides are very short fragments of the CCR5 protein and as such may not contain the gp120 binding site. You may wish to use the whole protein. While this is not currently available at Abcam, you may find this by searching biocompare.com These peptides (ab7876ab92719 and ab95861) are the immunizing antigens for antibodies ab7346, ab64620 and ab85837 respectively.



    None of the antibodies has yet been tested in ELISA. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

    The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



    If you would be interested in this offer please let me know.



    The product pages for these antibodies are at:

    https://www.abcam.com/CCR5-antibody-ab7346.html



    https://www.abcam.com/CCR5-antibody-mAbcam-64620-ab64620.html



    https://www.abcam.com/CCR5-antibody-ab85837.html



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Abcam Scientific Support

    回复于 May 18 2012

    Question

    Could you tell me if the epitope recognized by your CCR5 (ab7346) corresponding to amino acids 6-20 of Human CCR5 is also present in HIV Clade C population, please?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 08 2011

    Answer

    Thank you for contacting us with your question. I aligned the immunogen sequence SSPIYDINYYTSEPC against the HIV M:C (taxid 505186) protein database and no putative conserved domains were identified. I hope this information is helpful, but please let me know if you have further questions.

    Read More

    Abcam Scientific Support

    回复于 Apr 08 2011

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No CCR5 band, only background bands. SAMPLE Human sample from blood derived Mature Dendritic cells. Purified protein using Pierce M-PER reagent. PRIMARY ANTIBODY Primary Antibody: Abcam rabbit polyclonal CCR5 at 1:1000, diluted with 3:1 with TBS-T and NAP-Blocker and incubated at 4C overnight. Wash 30 minutes with TBS-T Attempted second time at advice of abcam tech, using 5:1 dilution of TBS-T and NAP-blocker. Wash 30 minutes with TBS-T DETECTION METHOD Detected with 45 minute step of Invitrogen Streptavadin 1:2500, then 30 minute wash. Developed with Pierce NBT-BCIP solution at RT until bands develop. POSITIVE AND NEGATIVE CONTROLS USED None ANTIBODY STORAGE CONDITIONS Stored at +4C SAMPLE PREPARATION Used 1:1 Laemmli Sample Buffer that has 5% B-MERC. Heated sample for 5 minutes at 95C. AMOUNT OF PROTEIN LOADED 30 micrograms per lane ELECTROPHORESIS/GEL CONDITIONS GeneMate Express Polyacrylamide Mini-gels. 4-20% Express gels TRANSFER AND BLOCKING CONDITIONS Transfer: Transfered at 25V for 100 minutes onto PVDF membrane. Blocker: G-Biosciences NAP-Blocker 1:1 with TBS-Tween. Blocked for 30 minutes at RT. SECONDARY ANTIBODY [another company] goat anti-rabbit, diluted 1:2000 in 3:1 TBS-T and NAP-blocker. Incubated at RT for 2 hours. Wash 30 minutes with TBS-T Attempted second time using 5:1 dilution of TBS-T and NAP-blocker. Wash 30 minutes with TBS-T HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I have changed the amount of blocking reagent in the Primary, secondary, and Streptavadin solutions from 3:1 TBS-T/NAP-Blocker to 5:1 TBS-T/NAP-Blocker with no different results.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 07 2006

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I appreciate the additional steps that you have performed in order to try and get this antibody to work. I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    回复于 Jun 08 2006

    Question

    This email is additional information for my previous email whichi subject is "Question about antibody and peptid". I forgot to ask you how pure they are and which method these peptides have been purified by. Thank you for your cooperation. ab2001, HREALEKDIKTSYIMDHC ab7346, SSPIYDINYYTSEPC ab3430, MSDFDSNPFADPDLNNPC ab3346, MSAPAQPPAEGTEGAAPGC ab3347, MSATAATVPPAAPAGEGGC Thank you for your cooperation

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2005

    Answer

    Thank you again for your enquiry. Regarding ab4969, ab4957, and ab4958 (the peptides corresponding to the antibodies you indicated), they are all greater than 95% pure, and they were purified via HPLC. If you have any further questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Apr 19 2005

    Question

    This email is additional information for my previous email whichi subject is "Question about antibody and peptid". I forgot to ask you how pure they are and which method these peptides have been purified by. Thank you for your cooperation. ab2001, HREALEKDIKTSYIMDHC ab7346, SSPIYDINYYTSEPC ab3430, MSDFDSNPFADPDLNNPC ab3346, MSAPAQPPAEGTEGAAPGC ab3347, MSATAATVPPAAPAGEGGC Thank you for your cooperation

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2005

    Answer

    Thank you for your enquiry. For ab2001 and ab7346, the peptides are non-HPLC, which averages out to 60-70% purity. Ab2001 (the antibody) was immunoaffinity chromatography purified IgG, and ab7346 (CCR5 antibody) is purified IgG. I will have to get back to you regarding the other peptides. If you have any additional questions, please let us know.

    Read More

    Abcam Scientific Support

    回复于 Apr 19 2005

    Question

    DESCRIPTION OF THE PROBLEM Multiple bands DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Can you send a pic of a WB indicating the correct size and location of the CCR5 band (s)?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 09 2004

    Answer

    We unfortunately do not have a picture of a Western to share, but the expected band size using ab7346 is approximately 40 kDa. To get rid of the non-specific bands on your blot, I suggest decreasing the concentrations of the primary and secondary and also make sure that you run a no primary control. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Mar 09 2004

    Question

    Could you tell me what cell line was used as a source of antigen in Western Blotting? Was a cell lysate used?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 27 2001

    Answer

    The Western blot was performed on THP-1 whole cell lysate.

    Read More

    Abcam Scientific Support

    回复于 Oct 01 2001

    Question

    Could you send me an example plot of human blood lymphocytes stained with your CCR5 antibody? (Flow Cytometry Plot)

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2001

    Answer

    This antibody has only been tested in Western blotting, and so we have no data on its us in flow cytometry.

    Read More

    Neil Hayward

    Abcam Scientific Support

    回复于 Jul 03 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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