Anti-CCR4抗体(ab1669)
Key features and details
- Goat polyclonal to CCR4
- Suitable for: WB
- Reacts with: Human, Cynomolgus monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CCR4抗体
参阅全部 CCR4 一抗 -
描述
山羊多克隆抗体to CCR4 -
宿主
Goat -
特异性
Peptide sequence is < 50 % identical to other human chemokine receptors in this region. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, Cynomolgus monkey -
免疫原
Synthetic peptide:
SNYYLYESIPKPCTKEGIKAFGE
, corresponding to amino acids 17-39 of Human CCR4 (extracellular domain). -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa). |
靶标
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功能
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
组织特异性
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1233 Human
- GenBank: NP_005499.1 Human
- Omim: 604836 Human
- SwissProt: P51679 Human
- Unigene: 184926 Human
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别名
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
图片
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Anti-CCR4 antibody (ab1669) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
CCR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab1669 被引用在 15 文献中.
- Xu W et al. E2F2 stimulates CCR4 expression and activates synovial fibroblast-like cells in rheumatoid arthritis. Cent Eur J Immunol 46:27-37 (2021). PubMed: 33897281
- Li L et al. Laminin ?2-mediating T cell exclusion attenuates response to anti-PD-1 therapy. Sci Adv 7:N/A (2021). PubMed: 33536206
- Li T et al. Graft IL-33 regulates infiltrating macrophages to protect against chronic rejection. J Clin Invest 130:5397-5412 (2020). PubMed: 32644975
- Yuan FY et al. Tanshinone IIA improves diabetes mellitus via the NF-?B-induced AMPK signal pathway. Exp Ther Med 16:4225-4231 (2018). PubMed: 30344697
- Wang Y et al. HIC1 deletion promotes breast cancer progression by activating tumor cell/fibroblast crosstalk. J Clin Invest 128:5235-5250 (2018). PubMed: 30204129