Anti-CCN3抗体(ab137677)
Key features and details
- Rabbit polyclonal to CCN3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CCN3抗体
参阅全部 CCN3 一抗 -
描述
兔多克隆抗体to CCN3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 58-357 of Human CCN3 (Uniprot ID: P48745).
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阳性对照
- Recombinant Human CCN3 protein (ab50076) can be used as a positive control in WB. 293T, A431, H1299, HeLaS2, Hela, HepG2, MOLT4, Mouse brain and Raji whole cell lysates; HeLa cells; Human gastric carcinoma N87 xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137677于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0)
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer (pH8.0) |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Immediate-early protein likely to play a role in cell growth regulation. -
组织特异性
Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. -
序列相似性
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4856 Human
- Entrez Gene: 18133 Mouse
- Omim: 164958 Human
- SwissProt: P48745 Human
- SwissProt: Q64299 Mouse
- Unigene: 235935 Human
- Unigene: 5167 Mouse
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别名
- CCN family member 3 antibody
- CCN3 antibody
- IBP-9 antibody
see all
图片
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Anti-CCN3 antibody (ab137677) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 39 kDa
12% SDS PAGE -
Anti-CCN3 antibody (ab137677) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 39 kDa
10% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling CCN3 using ab137677 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma N87 xenograft tissue, labelling CCN3 with ab137677 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab137677 被引用在 11 文献中.
- Yan X et al. Single nucleotide mutation changes the capability of CCN3 in osteosarcoma cell invasion and differentiation. Transl Oncol 24:101485 (2022). PubMed: 35858494
- Mizukawa T et al. RFX1-mediated CCN3 induction that may support chondrocyte survival under starved conditions. J Cell Physiol 236:6884-6896 (2021). PubMed: 33655492
- Henrot P et al. Decreased CCN3 in Systemic Sclerosis Endothelial Cells Contributes to Impaired Angiogenesis. J Invest Dermatol 140:1427-1434.e5 (2020). PubMed: 31954725
- Zhang S et al. E2F1/SNHG7/miR-186-5p/MMP2 axis modulates the proliferation and migration of vascular endothelial cell in atherosclerosis. Life Sci 257:118013 (2020). PubMed: 32603818
- Duan L et al. CCN3 Signaling Is Differently Regulated in Placental Diseases Preeclampsia and Abnormally Invasive Placenta. Front Endocrinol (Lausanne) 11:597549 (2020). PubMed: 33304321