Key features and details
- Rabbit polyclonal to CCL25
- Suitable for: WB, ELISA, Neutralising, Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
参阅全部 CCL25 一抗
经测试应用适用于: WB, ELISA, Neutralising, Sandwich ELISAmore details
Highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine)
- Recombinant human CCL25 protein (ab586) can be used as a positive control in WB.
This product was previously labelled as TECK
形式Lyophilized:Reconstitute with 200µl of sterile water.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent concentration. To detect hTECK by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTECK.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100.00 ng/ml), a concentration of 10.0 - 15.0 µg/ml of this antibody is required.|
|Sandwich ELISA||Use a concentration of 0.5 - 1 µg/ml.
Assuming 100µl/well, a concentration of 0.5-2.0 µg/ml of ab2164 will detect at least 0.2-0.4 ng/well of recombinant human CCL25 when used with ab83146 as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
功能Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.
组织特异性Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
序列相似性Belongs to the intercrine beta (chemokine CC) family.
- Information by UniProt
- A130072A22Rik antibody
- C-C motif chemokine 25 antibody
- CCL 25 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.