概述

  • 产品名称
  • 描述
    兔多克隆抗体to CCDC11
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (YSQRFGTVQR EVKGPTPKVV IVRSKPPKGQ GAEHHLERIR RSHQKHNAIL) of Human CCDC11 (NP_659457).

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).Can be blocked with CCDC11 peptide (ab117880). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-CCDC11 antibody (ab84324) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG

    at 1/50000 dilution

    Predicted band size: 62 kDa
    Observed band size: 62 kDa



    Gel concentration: 12%
  • ab84324 staining CCDC11 in human skin fibroblasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformladehyde, permeabilized using 0.25% Triton X-100/PBS, blocked with 5% serum for 30 minutes at 25°C and then incubated with ab84324 at 1µg/ml for 3 hours at 25°C. The secondary used was an FITC conjugated donkey anti-rabbit IgG (H+L) used at a 1/500 dilution. Nuclear staining with DAPI.

    See Abreview

文献

This product has been referenced in:
  • Perles Z  et al. A human laterality disorder associated with recessive CCDC11 mutation. J Med Genet 49:386-90 (2012). ICC/IF ; Human . Read more (PubMed: 22577226) »
See 1 Publication for this product

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin Fibroblasts)
Specification
Skin Fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 28 2011

Answer

Thank you for your message which has been forwarded to me as my colleague Miia is currently away from the office. We do request that customers submit their Abreview before the expirey date in order to make use of this offer. However, we understand this is not always possible. I will be pleased to extend the expirey date of discount code 100%ABR-E7763 in this case to 3rd Jan 2012. Please also reference the correspondence number of this email as well as the discount code when claiming the free antibody and submitting the Abreview: CCEE 3275159. This will mean my colleague will know that the expirey has been extended. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

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Answer

Thank you for contacting us. The blocking peptide for the Anti-CCDC11 antibody (ab84324) is ab117880. This was previously not in our catalogue but has now been added due to your request. This may not be possible in every case but if any of your customers are interested in blocking peptides that cannot be found through the website it is always worth getting in contact and it may be that we can add it such as in this case. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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