存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab84324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).Can be blocked with CCDC11 peptide (ab117880). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
- Information by UniProt
- 4933415I03Rik antibody
- CCD11_HUMAN antibody
- CCDC11 antibody
Anti-CCDC11 antibody (ab84324) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG
at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Gel concentration: 12%
ab84324 staining CCDC11 in human skin fibroblasts by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformladehyde, permeabilized using 0.25% Triton X-100/PBS, blocked with 5% serum for 30 minutes at 25°C and then incubated with ab84324 at 1µg/ml for 3 hours at 25°C. The secondary used was an FITC conjugated donkey anti-rabbit IgG (H+L) used at a 1/500 dilution. Nuclear staining with DAPI.