Application
Western blot
Sample
Sheep Tissue lysate - whole (Cardiac)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
10 µg
Specification
Cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 22°C · Diluent: 1% milk TBST
Dilution
1/3000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 15-20 kDa
Positive control
5 x samples of sheep cardiac tissue, lysed in RIPA with protease inhibitors
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 20 2013