重组PE Anti-Caveolin-1抗体[EPR15554] - N-terminal (ab212007)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR15554] to Caveolin-1 - N-terminal
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-Caveolin-1抗体[EPR15554] - N-terminal
参阅全部 Caveolin-1 一抗 -
描述
PE兔单克隆抗体[EPR15554] to Caveolin-1 - N-terminal -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Recombinant fragment within Human Caveolin-1 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q03135 -
阳性对照
- Flow Cyt (intra): A549 cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15554 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab212007于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
说明 |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
靶标
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功能
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
组织特异性
Expressed in muscle and lung, less so in liver, brain and kidney. -
疾病相关
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
序列相似性
Belongs to the caveolin family. -
翻译后修饰
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
细胞定位
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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数据库链接
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Omim: 601047 Human
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- Unigene: 74034 Human
- Unigene: 28278 Mouse
- Unigene: 470537 Mouse
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别名
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
图片
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Overlay histogram showing A549 cells stained with ab212007 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab212007, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab212007 尚未被引用在任何文献中。