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使用敲除细胞株进行验证

Anti-Caveolin-1抗体- Caveolae Marker (ab2910)

  • Datasheet
  • SDS
Reviews (55)Q&A (10)References (122)

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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

Key features and details

  • Rabbit polyclonal to Caveolin-1 - Caveolae Marker
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Caveolin-1抗体- Caveolae Marker
    参阅全部 Caveolin-1 一抗
  • 描述

    兔多克隆抗体to Caveolin-1 - Caveolae Marker
  • 宿主

    Rabbit
  • 特异性

    Detects caveolin-1.
  • Tested Applications & Species

    Application Species
    ICC
    Rat
    Human
    WB
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human Caveolin-1 aa 1-17.
    Sequence:

    MSGGKYVDSEGHLYTVP


    Database link: Q03135
    (Peptide available as ab4928)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Human lung, heart and spleen tissue lysates; Rat heart protein extract. ICC: HeLa and NIH/3T3 cells; Rat astrocytes.
  • 常规说明

    This antibody can be used as a marker for lipid raft fractions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Caveolin-1 peptide (ab4928)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Recombinant Protein

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
ICC
Rat
Human
WB
Rat
Human
All applications
Sheep
Rabbit
Horse
Cow
Cat
Pig
Chimpanzee
Gorilla
African green monkey
African bush elephant
应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
WB (22)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).
说明
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).

靶标

  • 功能

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • 组织特异性

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • 疾病相关

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 序列相似性

    Belongs to the caveolin family.
  • 翻译后修饰

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • 细胞定位

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession Q03135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 493668 Cat
    • Entrez Gene: 463670 Chimpanzee
    • Entrez Gene: 281040 Cow
    • Entrez Gene: 101125469 Gorilla
    • Entrez Gene: 857 Human
    • Entrez Gene: 404693 Pig
    • Entrez Gene: 100008837 Rabbit
    • Entrez Gene: 25404 Rat
    • Entrez Gene: 445468 Sheep
    • Omim: 601047 Human
    • SwissProt: A0M8S7 Cat
    • SwissProt: Q2QLF2 Chimpanzee
    • SwissProt: P79132 Cow
    • SwissProt: Q2IBF0 Gorilla
    • SwissProt: Q2QLB0 Horse
    • SwissProt: Q03135 Human
    • SwissProt: Q6RVA9 Pig
    • SwissProt: Q09YN6 Rabbit
    • SwissProt: P41350 Rat
    • SwissProt: Q6B3Y2 Sheep
    • Unigene: 74034 Human
    • Unigene: 22518 Rat
    see all
  • 别名

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

图片

  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml

    Lane 1 : Human lung
    Lane 2 : Human heart
    Lane 3 : Human spleen

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    ab2910 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab2910 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 2 µg/ml + Rat heart protein extract

    Predicted band size: 20 kDa

  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    Rat astrocytes stained with fluorescently labeled Caveolin-1 antibody.

    Primary antibody is ab2910 at a dilution of 1/500 and the secondary antibody is Texas red labeled anti-rabbit IgG at a dilution of 1/1000.

    This image was kindly supplied as part of the review submitted by Donghui Zhu.

  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)This image is courtesy of an anonymous Abreview

    ab2910 staining Caveolin-1 - Caveolae Marker in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (122)

发表研究结果有使用 ab2910?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2910 被引用在 122 文献中.

  • Cai X  et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). PubMed: 31190436
  • Martin GG  et al. High Glucose and Liver Fatty Acid Binding Protein Gene Ablation Differentially Impact Whole Body and Liver Phenotype in High-Fat Pair-Fed Mice. Lipids N/A:N/A (2020). PubMed: 32314395
  • Nie Y  et al. The Na/K-ATPase a1 and c-Src form signaling complex under native condition: A crosslinking approach. Sci Rep 10:6006 (2020). PubMed: 32265464
  • Barruet E  et al. Functionally heterogeneous human satellite cells identified by single cell RNA sequencing. Elife 9:N/A (2020). PubMed: 32234209
  • Hasan SS  et al. Endothelial Notch signaling controls insulin transport in muscle. EMBO Mol Med 12:e09271 (2020). PubMed: 32187826
View all Publications for this product

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1-10 of 55 Abreviews

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Oct 20 2020

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Oct 13 2020

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Fractions from mouse liver tissue to obtain lipid)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Fractions from mouse liver tissue to obtain lipid
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Apr 18 2019

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human Alveolar Epithelial cells (hAELVi))
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
1e+006 cells
Specification
human Alveolar Epithelial cells (hAELVi)
Blocking step
Western Breeze Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Apr 30 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human Alveolar Epithelial cells (hAELVi))
Permeabilization
Yes - Saponin
Specification
human Alveolar Epithelial cells (hAELVi)
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Apr 19 2018

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
50 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Juan Rodríguez Vita

Verified customer

提交于 Mar 27 2017

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 fibroblasts)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
3T3 fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Mar 01 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Myocardium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Myocardium
Blocking step
(agent) for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

提交于 Jan 28 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neuron)
Permeabilization
Yes - 0.2%Triton
Specification
neuron
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Oct 13 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS-7)
Permeabilization
Yes - 0.2%Triton
Specification
COS-7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More

Dr. Ya Zhou

Verified customer

提交于 Oct 06 2015

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