Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH 3T3)
Specification
NIH 3T3
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% NGS
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s)
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: Molecular probes goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
We have been looking for a suitable caveolae marker antibody to use in colocalization experiments in conjunction with a battery of mouse monoclonal antibodies. Compared with other polyclonal antibodies that we have used, we found that this antibody performed on par with the best monoclonal anti-caveolin-1 antibodies available. We are very pleased with this reagent, and recommend it to other investigators.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. William Ackerman
Verified customer
提交于 May 17 2006