Anti-Caveolin-1抗体[7C8] (ab17052)
Key features and details
- Mouse monoclonal [7C8] to Caveolin-1
- Suitable for: WB, ICC
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caveolin-1抗体[7C8]
参阅全部 Caveolin-1 一抗 -
描述
小鼠单克隆抗体[7C8] to Caveolin-1 -
宿主
Mouse -
特异性
The monoclonal antibody 7C8 recognizes caveolin-1a as well as caveolin-1ß, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat. -
经测试应用
适用于: WB, ICCmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Rat Caveolin-1. GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes (Sprague Dawley). The antibody recognises epitope between residue 32 and the C-terminus.
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阳性对照
- WB: A431, A549 and HUVEC cell lysates. ICC: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
0.2 µm filtered -
克隆
单克隆 -
克隆编号
7C8 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa.
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ICC |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa. |
ICC
Use at an assay dependent concentration. |
靶标
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功能
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
组织特异性
Expressed in muscle and lung, less so in liver, brain and kidney. -
疾病相关
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
序列相似性
Belongs to the caveolin family. -
翻译后修饰
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
细胞定位
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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数据库链接
- Entrez Gene: 857 Human
- Omim: 601047 Human
- SwissProt: Q03135 Human
- Unigene: 74034 Human
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别名
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
图片
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All lanes : Anti-Caveolin-1 antibody [7C8] (ab17052) at 1 µg/ml
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 21-24 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab17052 observed at 21-24 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab17052 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab17052 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab17052 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab17052 at 1/500 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
ab17052 staining Caveolin-1 in human Hacat keratinocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/75 for 24 hours at 4°C. The secondary antibody used was conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.
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Anti-Caveolin-1 antibody [7C8] (ab17052) at 1/500 dilution + HUVEC whole cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
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Immunocytochemical analysis of Mouse Bend.3 cells, labeling Caveolin-1 with ab17052. Cells were paraformaldehyde fixed, permeabilized with PBS + 0.1% Triton (PBT), and blocked with 10% serum for 1 hour at 22°C. Staining with ab17052 (diluted 1/200) was for 16 hours at 4°C.
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ab17052 at a 1/500 dilution staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody. Bound antibody was detected using a FITC conjugated goat anti-mouse antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (35)
ab17052 被引用在 35 文献中.
- Tian Y et al. Role of Caveolae in the Development of Microvascular Dysfunction and Hyperglycemia in Type 2 Diabetes. Front Physiol 13:825018 (2022). PubMed: 35250626
- Yeung CC et al. Circadian regulation of protein cargo in extracellular vesicles. Sci Adv 8:eabc9061 (2022). PubMed: 35394844
- Li A et al. Mechanical properties of tunneling nanotube and its mechanical stability in human embryonic kidney cells. Front Cell Dev Biol 10:955676 (2022). PubMed: 36238686
- Kearney AL et al. Akt phosphorylates insulin receptor substrate to limit PI3K-mediated PIP3 synthesis. Elife 10:N/A (2021). PubMed: 34253290
- van der Horst J et al. Dynein regulates Kv7.4 channel trafficking from the cell membrane. J Gen Physiol 153:N/A (2021). PubMed: 33533890