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Neuroscience Cell Adhesion Proteins Membrane Proteins

Anti-Cathepsin L + V抗体[33/2] (ab6314)

  • Datasheet
Reviews (5)Q&A (12)References (30)

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Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
  • Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314)
  • Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
  • Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)

Key features and details

  • Mouse monoclonal [33/2] to Cathepsin L + V
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Cathepsin L + V抗体[33/2]
    参阅全部 Cathepsin L + V 一抗
  • 描述

    小鼠单克隆抗体[33/2] to Cathepsin L + V
  • 宿主

    Mouse
  • 特异性

    Reacts specifically with the native and denatured forms of human cathepsin L (25kD) and procathepsin L (42 kD). Does not react with human cathepsin types B1, D1, H and S1, procathepsins B and D1 and recombinant procathepsin H, rat cathepsin B1, and mouse procathepsins B1 and D1.
  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • 表位

    Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
  • 阳性对照

    • WB: Human and Mouse lung tissue lysates. Human, Mouse and Rat kidney tissue lysates. IHC-P: FFPE Human colon adenocarcinoma tissue sections.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    33/2
  • 同种型

    IgG1
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • HRP Anti-Cathepsin L + V antibody [33/2] (ab197278)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6314于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 38 kDa). Abcam recommends using 3% milk as the blocking agent.
IHC-P (1)
Use at an assay dependent concentration.
说明
WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 38 kDa). Abcam recommends using 3% milk as the blocking agent.
IHC-P
Use at an assay dependent concentration.
应用说明
Is unsuitable for Flow Cyt.

图片

  • Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
    Lane 2 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
    Lane 3 : Anti-His tag at 1/10000 dilution

    All lanes : Human Cathepsin L recombinant protein fraction

    Secondary
    Lanes 1 & 3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
    Lane 2 : Rabbit Anti-Mouse secondary ab at 1/2000 dilution

    Predicted band size: 38 kDa



    Blocking buffer and concentration:5% NFDM/TBST

    Diluting buffer and concentration:5% NFDM/TBST

    Observed MW:30

    Exposure time:3 minutes

  • Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
    Lane 2 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/100 dilution
    Lane 3 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/500 dilution
    Lane 4 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
    Lane 5 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
    Lane 6 : Anti-His tag at 1/10000 dilution

    All lanes : Human Cathepsin V recombinant protein fraction

    Secondary
    Lanes 1-3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
    Lanes 4-5 : Rabbit Anti-Mouse secondary ab at 1/2000 dilution
    Lane 6 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer and concentration:5% NFDM/TBST

    Diluting buffer and concentration:5% NFDM/TBST

    Observed MW:30

    Exposure time:3 minutes

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314)

    IHC image of Cathepsin L staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6314, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    All lanes : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution (3% milk block)

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Kidney (Mouse) Tissue Lysate
    Lane 5 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?
    Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
    Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)Image courtesy of Ratnakar Singh by Abreview.
    Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/750 dilution + whole cell lysate prepared from U87MG human glioblastoma at 50 µg

    Secondary
    Goat anti-mouse HRP at 1/2000 dilution

    Predicted band size: 38 kDa

    See Abreview

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (30)

发表研究结果有使用 ab6314?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6314 被引用在 30 文献中.

  • Sankpal NV  et al. Cancer-associated mutations reveal a novel role for EpCAM as an inhibitor of cathepsin-L and tumor cell invasion. BMC Cancer 21:541 (2021). PubMed: 33980181
  • Bass JJ  et al. The mechanisms of skeletal muscle atrophy in response to transient knockdown of the vitamin D receptor in vivo. J Physiol 599:963-979 (2021). PubMed: 33258480
  • Shen S  et al. BRD4 Inhibition Protects Against Acute Pancreatitis Through Restoring Impaired Autophagic Flux. Front Pharmacol 11:618 (2020). PubMed: 32457617
  • Li Z  et al. Enhanced lysosomal function is critical for paclitaxel resistance in cancer cells: reversed by artesunate. Acta Pharmacol Sin N/A:N/A (2020). PubMed: 32704040
  • Fukuda M  et al. Disruption of P2X4 purinoceptor and suppression of the inflammation associated with cerebral aneurysm formation. J Neurosurg N/A:1-13 (2019). PubMed: 31860812
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 17 Abreviews or Q&A

Western blot abreview for Anti-Cathepsin L antibody [33/2]

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
80 µg
Specification
macrophage cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Oct 30 2015

Western blot abreview for Anti-Cathepsin L antibody [33/2]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (kidney, lung)
Specification
kidney, lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

提交于 Dec 22 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cathepsin L antibody [33/2]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mammary gland)
Specification
mammary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 3.2% · Temperature: 22°C
Read More

Dr. Irina Teplova

Verified customer

提交于 Jul 19 2011

Western blot abreview for Anti-Cathepsin L antibody [33/2]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Loading amount
50 µg
Specification
Glioblastoma
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 30°C
Read More

Dr. Ratnakar Singh

Verified customer

提交于 Jun 10 2011

Western blot abreview for Anti-Cathepsin L antibody [33/2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA MB 231 cells)
Loading amount
10 µg
Specification
MDA MB 231 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Apr 09 2009

Question

Cusotmer kindly called as a recently purchased vial of this product was not working as stated on datasheet.

Read More

Abcam community

Verified customer

Asked on Aug 14 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Abcam Scientific Support

回复于 Aug 14 2012

Question

High background with these antibodies in IHC-Fr on human-derived tumors grown in mouse.

Read More

Abcam community

Verified customer

Asked on Apr 25 2012

Answer

Thank you for your call today and for letting us know about the trouble with these antibodies.

I'm sending a free of charge vial of ab826, which should arrive tomorrow on the order ***. The vials of ab30443 and ab58991 will be arriving early next week on the order ***. Please keep me updated about how these antibodies work in your staining.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you. Have a nice day.

Read More

Abcam Scientific Support

回复于 Apr 25 2012

Question

I'm using this antibody to stain PFA fixed rat cells, but it's staining the cell membrane and not lysosomes.

Read More

Abcam community

Verified customer

Asked on Apr 04 2012

Answer

Thank you for contacting us.


I am sorry that this antibody did not perform as stated on the datasheet. We will be refunding the purchase of this product to your credit card.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Abcam Scientific Support

回复于 Apr 04 2012

Question

Thank you for your response.   Our customer has responded with the following;   Thanks for your response. We decide to order AB77179 (Anti-Entactin antibody), AB6314 (Anti-Cathepsin L antibody), AB38975 (Anti-TIMP2 antibody) and AB41684 (Anti-Cyclophilin A antibody) with testing discount. Could you please provide discount code to me. If you could kindly provide the codes soon that would be great.  Enjoy your day Kind Regards        

Read More

Abcam community

Verified customer

Asked on Nov 08 2011

Answer

Thank you for your message and for confirming these details. I am very pleased to hear the customer would like to accept our offer and test these antibodies in hamster. ab41684 Discount code: #### ab38975 Discount code: #### ab6314 Discount code: #### ab77179 Discount code: #### Expirey date for all these codes: 7th March 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit the Abreviews for hamster samples and include the relevant code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreviews and wish the customer luck with their research.

Read More

Abcam Scientific Support

回复于 Nov 08 2011

Question

Hi,  I hope you are well. The customer has responded with the following;  I finally have time to find the protein sequence and get back to you. Could someone help me to check whether these three antibodies have testing discount (Entactin, Cathepsin B and L)? The comment is attached in the email. The sequence for Cathepsin B and L are also in the word document. Although I couldn’t find the sequence of Entactin, we still would like to try it. Thanks a lot. See attached a document showing the sequences.   AB77179 AB6314 AB16045   Is our customer eligible for the discount of these 3 antibodies?   I look forward to your response.     Kind Regards  

Read More

Abcam community

Verified customer

Asked on Oct 31 2011

Answer

Thank you for your message which has been forwarded to me as Karin is currently away from the office. I have reviewed these three antibodies with respect to discount codes: ab77179 I am sorry I am also unable to check the alignment of the immunogen with the hamster sequence, because there is regrettably no hamster sequence available on the protein database sites. I would be pleased to make an exception and provide a discount code in this case anyway, to test in Hamster. ab6314 There is 73% Alignment of the human full length immunogen to the hamster sequence you have provided. This is a little low, but there may be some crossreactivity so I would be pleased to provide a discount. ab16045 There is 14% alignment of the hamster sequence provided with the immunogen. This is very low so the antibody will probably not detect in hamster, and so I am sorry we would be unable to provide a discount for this particular antibody to test in hamster. I hope this will be helpful to you. Please confirm if the customer would like to receive a code for testing ab6314 and ab77179 in hamster and I will be pleased to arrange this for you.

Read More

Abcam Scientific Support

回复于 Oct 31 2011

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