为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Neuroscience Cell Adhesion Proteins Membrane Proteins

Anti-Cathepsin D抗体[CTD-19] (ab6313)

  • Datasheet
  • SDS
Reviews (16)Q&A (11)References (47)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
  • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)

Key features and details

  • Mouse monoclonal [CTD-19] to Cathepsin D
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

你可能会感兴趣

一抗
Product image
Anti-CaSR antibody (ab137408)
蛋白
Recombinant Mouse Cathepsin D protein (ab191943)
一抗
Product image
Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab192489)

查看更多相关产品

概述

  • 产品名称

    Anti-Cathepsin D抗体[CTD-19]
    参阅全部 Cathepsin D 一抗
  • 描述

    小鼠单克隆抗体[CTD-19] to Cathepsin D
  • 宿主

    Mouse
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Cathepsin D.
    Database link: P07339

  • 阳性对照

    • Human breast carcinoma tissue or cell line extract.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.097% Sodium azide
  • Concentration information loading...
  • 纯度

    Proprietary Purification
  • 纯化说明

    Purified from TCS
  • 克隆

    单克隆
  • 克隆编号

    CTD-19
  • 同种型

    IgG2a
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Cysteine protease
    • Cathepsins

相关产品

  • Assay kits

    • Cathepsin D Inhibitor Assay Kit (ab126779)
    • Cathepsin D Activity Assay Kit (Fluorometric) (ab65302)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Mouse Cathepsin D protein (ab191943)

应用

Our Abpromise guarantee covers the use of ab6313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa.

靶标

  • 功能

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 组织特异性

    Expressed in the aorta extrcellular space (at protein level).
  • 疾病相关

    Ceroid lipofuscinosis, neuronal, 10
  • 序列相似性

    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻译后修饰

    N- and O-glycosylated.
  • 细胞定位

    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Target information above from: UniProt accession P07339 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1509 Human
    • Entrez Gene: 13033 Mouse
    • Omim: 116840 Human
    • SwissProt: P07339 Human
    • SwissProt: P18242 Mouse
    • Unigene: 654447 Human
    • Unigene: 231395 Mouse
    • 别名

      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    图片

    • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)This image is courtesy of an anonymous Abreview
      Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg

      Secondary
      HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 28,48,50 kDa
      why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      Blocked with 5% milk for 1 hour at 22°C.

      Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.

      This image was generated using the ascites version of the product.

      See Abreview

    • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells


      This image was generated using the ascites version of the product.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (47)

    发表研究结果有使用 ab6313?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6313 被引用在 47 文献中.

    • Kim SY  et al. Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 9:N/A (2020). PubMed: 32131492
    • Wang LL  et al. Downregulation of long noncoding RNA LINC01419 inhibits cell migration, invasion, and tumor growth and promotes autophagy via inactivation of the PI3K/Akt1/mTOR pathway in gastric cancer. Ther Adv Med Oncol 11:1758835919874651 (2019). PubMed: 31579114
    • Chrzanowski U  et al. Evaluation strategy to determine reliable demyelination in the cuprizone model. Metab Brain Dis 34:681-685 (2019). PubMed: 30607821
    • Koike S & Jahn R SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. Nat Commun 10:1608 (2019). PubMed: 30962439
    • Pauwels AM  et al. Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation. Mol Cell Proteomics 18:909-922 (2019). PubMed: 30808727
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 27 Abreviews or Q&A

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    25 µg
    Treatment
    5 µg/ml LPS, 24 h
    Specification
    cardiomyocytes
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Barbara Thaler

    Verified customer

    提交于 Sep 16 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Treatment
    1 µg/ml LPS, 24 h
    Specification
    cardiomyocytes
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Barbara Thaler

    Verified customer

    提交于 Sep 16 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (macrophages)
    Permeabilization
    Yes - 0.1% triton
    Specification
    macrophages
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 18 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    提交于 Jul 09 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (CSF)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    CSF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 11 2015

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain tissue)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 11 2015

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Sample
    Human Cell lysate - other (Glioblastoma cell lines)
    Specification
    Glioblastoma cell lines
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Mr. Achilleas Mitrakas

    Verified customer

    提交于 Apr 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (DU145 Human prostate)
    Specification
    DU145 Human prostate
    Permeabilization
    Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
    Fixative
    Paraformaldehyde
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    提交于 Nov 21 2013

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (adipocyte tissue)
    Loading amount
    35 µg
    Specification
    adipocyte tissue
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 18 2012

    Question

    Sehr geehrter Herr Hryca,
    vielen lieben Dank für das Angebot. Wir würden gerne die Maus-Antikörper
    bestellen und dabei ist uns aufgefallen, dass wir keine Möglichkeit einer
    Positivkontrolle haben. Können Sie uns da vielleicht weiterhelfen?
    Mit freundlichen Grüßen aus Düsseldorf

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Vielen Dank für Ihre Anfrage die Herr Hryca uns in den technischen Dienst weitergeleitet hat.

    Ich habe geschaut, und wir haben viele möglichen positiven Kontrollen für Sie. Können Sie mir bitte noch sagen, welche Art von positive Kontrolle Sie benötigen? Ist es ein Lysat für Western blot oder ein Gewebeschnitt für Immunohistochemie? Haben Sie auch eine Präferenz für eine Spezies (Maus, human)?

    Hier mal eine Aufstellung von möglichen positiven Kontrollen. Ich freue mich wieder von Ihnen zu hören, um Ihnen genaure Vorschläge machen zu können.




    cell lines Slide
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=2105 Rabbit polyclonal to IL1 beta LPS treated RAW 264.7 cells, Alveolar macrophages (MHS cell line) liver, diseased, inflammed tissue, monocytes, macrophages
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=6313 Mouse monoclonal [CTD-19] to Cathepsin D SKBR3, MCF7 Human liver tissue.
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=6535 Mouse monoclonal [BRM-22] to Hsp70 Hela, 293T,MCF7, Jurkat, HepG2, A431, HCT116, PC3, ... liver, brain, testes, heart, skeletal muscle
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=8219 Mouse monoclonal [MEM-259] to CD63 HT1080, HepG2, OVCAR-3, HL60 and A375 hemapoietic ells, tissue macrophages, platelet, human melanoma tissue, monocytes, macrophages, endothelial cells, SM cells, brain white matter, peripheral nerves
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=25631 Mouse monoclonal [H4B4] to LAMP2 - Lysosome Marker HepG2, 3T3, HeLa, RAW264.7, ... placenta, lung, liver
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=50533 Mouse monoclonal [Rab7-117] to RAB7 HepG2, B16, SK-MEL-28, 3T3, Human brain whole tissue, mouse neuroblastoma
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=68340 Mouse monoclonal to Rab9 Hela, A172, 3T3, 293, PC-12. COS-7, THP-1 tonsil, spleen, prostate cancer, stomach tissu
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=72907 Rabbit polyclonal to Rab5b Hela, Jurkat, HEK293, HepG2 Human Placenta Tissue, Human Brain Tissue.

    Read More

    Abcam Scientific Support

    回复于 Aug 14 2012

    1-10 of 27 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 3
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    ?ICP?14046373?-1

    ????? 31011502007413?

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有