Anti-Cathepsin D抗体[CTD-19] (ab6313)
![Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.cn/ps/products/6/ab6313/Images/ab6313-275029-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg "Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)")
Key features and details
- Mouse monoclonal [CTD-19] to Cathepsin D
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Cathepsin D抗体[CTD-19]
参阅全部 Cathepsin D 一抗 -
描述
小鼠单克隆抗体[CTD-19] to Cathepsin D -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human Cathepsin D.
Database link: P07339 -
阳性对照
- Human breast carcinoma tissue or cell line extract.
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常规说明
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.097% Sodium azide -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
CTD-19 -
同种型
IgG2a -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (12) |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa. |
靶标
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功能
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
组织特异性
Expressed in the aorta extrcellular space (at protein level). -
疾病相关
Ceroid lipofuscinosis, neuronal, 10 -
序列相似性
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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数据库链接
- Entrez Gene: 1509 Human
- Omim: 116840 Human
- SwissProt: P07339 Human
- Unigene: 654447 Human
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别名
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
see all
图片
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Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)This image is courtesy of an anonymous AbreviewAnti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg
Secondary
HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28,48,50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.cn/ps/products/6/ab6313/Images/ab6313-12229-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg)
Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cellsThis image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab6313 被引用在 54 文献中.
- Castroflorio E et al. The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. Cell Mol Life Sci 78:3503-3524 (2021). PubMed: 33340069
- Kim SY et al. Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 9:N/A (2020). PubMed: 32131492
- Feng Y et al. Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. Cell Death Dis 11:513 (2020). PubMed: 32641688
- Ghosh S et al. ß-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway. Cell 183:1520-1535.e14 (2020). PubMed: 33157038
- Jia J et al. AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal Damage via a Novel Galectin-Directed Ubiquitin Signal Transduction System. Mol Cell 77:951-969.e9 (2020). PubMed: 31995728