Anti-Cathepsin D抗体[CTD-19] (ab6313)
![Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.cn/ps/products/6/ab6313/Images/ab6313-275029-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg "Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)")
Key features and details
- Mouse monoclonal [CTD-19] to Cathepsin D
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
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产品名称
Anti-Cathepsin D抗体[CTD-19]
参阅全部 Cathepsin D 一抗 -
描述
小鼠单克隆抗体[CTD-19] to Cathepsin D -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Full length native protein (purified) corresponding to Human Cathepsin D.
Database link: P07339 -
阳性对照
- Human breast carcinoma tissue or cell line extract.
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常规说明
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.097% Sodium azide -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
CTD-19 -
同种型
IgG2a -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab6313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 52 kDa. |
靶标
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功能
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
组织特异性
Expressed in the aorta extrcellular space (at protein level). -
疾病相关
Ceroid lipofuscinosis, neuronal, 10 -
序列相似性
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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数据库链接
- Entrez Gene: 1509 Human
- Entrez Gene: 13033 Mouse
- Omim: 116840 Human
- SwissProt: P07339 Human
- SwissProt: P18242 Mouse
- Unigene: 654447 Human
- Unigene: 231395 Mouse
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别名
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
see all
图片
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Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)This image is courtesy of an anonymous AbreviewAnti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg
Secondary
HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28,48,50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.cn/ps/products/6/ab6313/Images/ab6313-12229-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg)
Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cellsThis image was generated using the ascites version of the product.
实验方案
文献 (47)
ab6313 被引用在 47 文献中.
- Kim SY et al. Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 9:N/A (2020). PubMed: 32131492
- Wang LL et al. Downregulation of long noncoding RNA LINC01419 inhibits cell migration, invasion, and tumor growth and promotes autophagy via inactivation of the PI3K/Akt1/mTOR pathway in gastric cancer. Ther Adv Med Oncol 11:1758835919874651 (2019). PubMed: 31579114
- Chrzanowski U et al. Evaluation strategy to determine reliable demyelination in the cuprizone model. Metab Brain Dis 34:681-685 (2019). PubMed: 30607821
- Koike S & Jahn R SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. Nat Commun 10:1608 (2019). PubMed: 30962439
- Pauwels AM et al. Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation. Mol Cell Proteomics 18:909-922 (2019). PubMed: 30808727
