Cathepsin B抑制剂Screening试剂盒(Fluorometric) (ab185438)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
概述
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产品名称
Cathepsin B抑制剂Screening试剂盒(Fluorometric)
参阅全部 Cathepsin B 试剂盒 -
检测方法
Fluorescent -
样品类型
Inhibitor compounds -
检测类型
Semi-quantitative -
产品概述
Abcam's Cathepsin B Inhibitor Screening Kit (ab185438) utilizes the ability of Cathepsin B to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin B-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This high-throughput adaptable assay kit is simple, sensitive, and rapid tool to screen the potential inhibitors of Cathepsin B.
Visit our FAQs page for tips and troubleshooting.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K147 Cathepsin B (CTSB) Inhibitor Screening Kit (Fluorometric). K147-100 is the same size as the 100 test size of ab185438.
Cathepsin B (CTSB, EC 3.4.22.1) is a lysosomal cysteine proteinase that is suggested to participate in intracellular degradation and turnover of proteins. It has also been implicated in tumor invasion and metastasis.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests 100 tests Cathepsin B Assay Buffer 1 x 15ml 1 x 15ml Cathepsin Inhibitor 1 x 20µl 1 x 20µl Cathepsin B Substrate 1 x 0.2ml 1 x 0.2ml DTT I 1 x 150µl 1 x 150µl Human Cathepsin B 1 x 5µl 1 x 5µl -
研究领域
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相关性
Cathepsin B is a normal lysosomal proteinase that is expressed in all cells. It is a cysteine endoproteinase structurally and functionally related to the papain family of proteases. Cathepsin B is synthesized as an inactive pro enzyme which, by removal of a 62 amino acid propeptide, is activated to the single chain form of 31 kDa or the two chain form (25 and 5 kDa subunits). Matured Cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins at acidic and neutral pH. Endogenous inhibitors of cysteine proteases, the cystatins and stefins, may play a major role in regulating Cathepsin B activity. In tumor cells and in cells exposed to mitogens, cathepsin B displays altered cellular trafficking resulting in the secretion of the 43 kDa precursor. Elevated levels of Cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers. Cathepsin B has also been implicated in the pathogenesis of rhematoid arthritis, muscular dystrophy and tumour metastasis. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). -
细胞定位
Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. -
别名
- Amyloid precursor protein secretase
- APP secretase
- APPS
see all
相关产品
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Related Products
图片
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Inhibition of Cathepsin B activity by the CTSB control
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Inhibition of Cathepsin B activity by the Cathepsin B inhibitor E-64
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Inhibition of Cathepsin B activity by the Cathepsin B inhibitor Chymostatin
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Inhibition of Cathepsin B activity by the Cathepsin B inhibitor Antipain dihydrochloride
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Inhibition of Cathepsin B activity by Cathepsin B Inhibitor Screening Kit was performed following kit protocol. This is example data only.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab185438 被引用在 4 文献中.
- Bai B et al. Peptidomimetic a-Acyloxymethylketone Warheads with Six-Membered Lactam P1 Glutamine Mimic: SARS-CoV-2 3CL Protease Inhibition, Coronavirus Antiviral Activity, and in Vitro Biological Stability. J Med Chem 65:2905-2925 (2022). PubMed: 34242027
- Li X et al. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. Theranostics 11:9821-9832 (2021). PubMed: 34815788
- Gautam J et al. Down-regulation of cathepsin S and matrix metalloproteinase-9 via Src, a non-receptor tyrosine kinase, suppresses triple-negative breast cancer growth and metastasis. Exp Mol Med 50:1-14 (2018). PubMed: 30185799
- Zhang X et al. Design, Synthesis, and Structure-Activity Relationship Study of Epoxysuccinyl-Peptide Derivatives as Cathepsin B Inhibitors. Biol Pharm Bull 40:1240-1246 (2017). PubMed: 28502922