Anti-Cathelicidin/CLP抗体(ab69484)
Key features and details
- Rabbit polyclonal to Cathelicidin/CLP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cathelicidin/CLP抗体
参阅全部 Cathelicidin/CLP 一抗 -
描述
兔多克隆抗体to Cathelicidin/CLP -
宿主
Rabbit -
特异性
There is 71% homology between the immunogen and mouse cathelicidin/CLP. This antibody has been published (see PubMed: 21724991) with mouse samples, but we were unable to see an appropriately sized band in mouse spleen or small intestine by WB. It may be that certain lots have worked with mouse, but we are unable to guarantee cross-reactivity with mouse after our testing.
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经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Cathelicidin/CLP aa 50-100 (internal sequence). The exact sequence is proprietary.
Database link: P49913 -
阳性对照
- Human spleen tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69484于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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说明 |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
靶标
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功能
Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity. -
组织特异性
Expressed in bone marrow and testis and neutrophils. -
序列相似性
Belongs to the cathelicidin family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 820 Human
- Omim: 600474 Human
- SwissProt: P49913 Human
- Unigene: 51120 Human
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别名
- 18 kDa cationic antimicrobial protein antibody
- Antibacterial peptide LL-37 antibody
- Antibacterial protein FALL-39 antibody
see all
图片
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ab69484 at 5 µg/ml staining Cathelicidin/CLP in human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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Anti-Cathelicidin/CLP antibody (ab69484) at 1 µg/ml + Human spleen tissue lysate at 15 µg
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Immunofluorescence of Cathelicidin/CLP in Human Spleen cells using ab69484 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab69484 被引用在 9 文献中.
- Smith KJ et al. The antimicrobial peptide cathelicidin drives development of experimental autoimmune encephalomyelitis in mice by affecting Th17 differentiation. PLoS Biol 20:e3001554 (2022). PubMed: 36026478
- Porter RJ et al. Colonic epithelial cathelicidin (LL-37) expression intensity is associated with progression of colorectal cancer and presence of CD8+ T cell infiltrate. J Pathol Clin Res 7:495-506 (2021). PubMed: 33988317
- Chen X et al. Human cathelicidin antimicrobial peptide suppresses proliferation, migration and invasion of oral carcinoma HSC-3 cells via a novel mechanism involving caspase-3 mediated apoptosis. Mol Med Rep 22:5243-5250 (2020). PubMed: 33174023
- Cao Y et al. LL-37 promotes neutrophil extracellular trap formation in chronic rhinosinusitis with nasal polyps. Clin Exp Allergy 49:990-999 (2019). PubMed: 31046155
- Chen S et al. Eluted 25-hydroxyvitamin D3 from radially aligned nanofiber scaffolds enhances cathelicidin production while reducing inflammatory response in human immune system-engrafted mice. Acta Biomater 97:187-199 (2019). PubMed: 31386930
- Lazzaretto B & Fadeel B Intra- and Extracellular Degradation of Neutrophil Extracellular Traps by Macrophages and Dendritic Cells. J Immunol 203:2276-2290 (2019). PubMed: 31519860
- Harman RM et al. Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds. Stem Cell Res Ther 8:157 (2017). PubMed: 28676123
- Li S et al. Exposure to fermentation supernatant of Staphylococcus aureus accelerated dedifferentiation of chondrocytes and production of antimicrobial peptides. J Orthop Res N/A:N/A (2017). PubMed: 28513981
- Douda DN et al. Innate immune collectin surfactant protein d simultaneously binds both neutrophil extracellular traps and carbohydrate ligands and promotes bacterial trapping. J Immunol 187:1856-65 (2011). WB ; Mouse . PubMed: 21724991