Anti-Catalase抗体- Peroxisome Marker (ab16731)
Key features and details
- Rabbit polyclonal to Catalase - Peroxisome Marker
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Catalase抗体- Peroxisome Marker
参阅全部 Catalase 一抗 -
描述
兔多克隆抗体to Catalase - Peroxisome Marker -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Goat, Dog, Ferret, Macaque monkey, Orangutan -
免疫原
Recombinant full length protein. purified from E.coli
Database link: P04040 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.03% Sodium azide
Constituents: HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16731于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-Fr | (3) |
Use at an assay dependent concentration.
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ICC/IF | (5) |
Use at an assay dependent concentration. See Abreview.
|
IHC-P | (2) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
WB | (14) |
1/2000. Predicted molecular weight: 60 kDa.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/2000. Predicted molecular weight: 60 kDa. |
靶标
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功能
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
疾病相关
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
序列相似性
Belongs to the catalase family. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 403474 Dog
- Entrez Gene: 847 Human
- Entrez Gene: 12359 Mouse
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: O97492 Dog
- SwissProt: P04040 Human
- SwissProt: P24270 Mouse
see all -
别名
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
图片
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Western Blot analysis of cell lysates.
Lane 1: HeLa cell lysates
Lane 2: Jurkat cell lysates
Lane 3: Mouse brain
Lane 4: Rat brainThe band marked with NS is probably non-specific.
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ICC/IF image of ab16731 stained Hela cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16731, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Ab16731 staining human normal adrenal gland tissue. Staining is localised to intracellular compartment (peroxisomes).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification -
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab16731) at 1/2000 dilution
Lane 1 : 40ug supernatant of mouse liver homogenate
Lane 2 : 20ug supernatant of mouse liver homogenate
Lane 3 : 5ug supernatant of mouse liver homogenate
Secondary
All lanes : HRP conjugated donkey anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 1 minute
This image is courtesy of an Abreview submitted by Sandra Sobocanec on 16 March 2006. -
ab16731 at 1/200 dilution staining Catalase in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used undiluted as secondary.
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ab16731 at a 1/200 dilution staining Catalase in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence, incubated for 9 hours at 4°C. Formalin fixed. Blocked with 2% BSA for 30 minutes at 20°C. Secondary used at 1/200 dilution polyclonal Goat anti-rabbit IgG conjugated to Alexa Fluor 488 (green). Nuclei stained with DAPI (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (171)
ab16731 被引用在 171 文献中.
- Brunetta HS et al. Nitrate consumption preserves HFD-induced skeletal muscle mitochondrial ADP sensitivity and lysine acetylation: A potential role for SIRT1. Redox Biol 52:102307 (2022). WB ; Mouse, Human . PubMed: 35398714
- Borsani E et al. Role of melatonin in autism spectrum disorders in a male murine transgenic model: Study in the prefrontal cortex. J Neurosci Res 100:780-797 (2022). PubMed: 35043490
- Oka S et al. Endogenous ROS production in early differentiation state suppresses endoderm differentiation via transient FOXC1 expression. Cell Death Discov 8:150 (2022). PubMed: 35365611
- Marković Filipović J et al. Effect of Acrylamide Treatment on Cyp2e1 Expression and Redox Status in Rat Hepatocytes. Int J Mol Sci 23:N/A (2022). PubMed: 35682741
- Amioka N et al. Pemafibrate Prevents Rupture of Angiotensin II-Induced Abdominal Aortic Aneurysms. Front Cardiovasc Med 9:904215 (2022). PubMed: 35845076