重组Anti-Caspr2/CNTNAP2抗体[EPR8738] (ab137052)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8738] to Caspr2/CNTNAP2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Caspr2/CNTNAP2抗体[EPR8738]
参阅全部 Caspr2/CNTNAP2 一抗 -
描述
兔单克隆抗体[EPR8738] to Caspr2/CNTNAP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fetal brain and Human glioma lysates; Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8738 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).
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IHC-P | (3) |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000. Detects a band of approximately 152 kDa (predicted molecular weight: 148 kDa). |
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. -
组织特异性
Predominantly expressed in nervous system. -
疾病相关
Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2. -
序列相似性
Belongs to the neurexin family.
Contains 2 EGF-like domains.
Contains 1 F5/8 type C domain.
Contains 1 fibrinogen C-terminal domain.
Contains 4 laminin G-like domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 26047 Human
- Entrez Gene: 66797 Mouse
- Omim: 604569 Human
- SwissProt: Q9UHC6 Human
- SwissProt: Q9CPW0 Mouse
- Unigene: 655684 Human
- Unigene: 440084 Mouse
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别名
- AUTS15 antibody
- CDFE antibody
- Cell recognition molecule Caspr2 antibody
see all
图片
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Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab137052 at a working dilution of 1 in 2000. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Caspr2/CNTNAP2 with unpurified ab137052 at dilution 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (purified) + Human glioma tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 148 kDa
Observed band size: 152 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 148 kDa
Observed band size: 152 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Rat brain tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 148 kDa
Observed band size: 152 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/5000 dilution (purified) + Mouse brain tissue lysate at 10 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 148 kDa
Observed band size: 152 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-Caspr2/CNTNAP2 antibody [EPR8738] (ab137052) at 1/1000 dilution (unpurified)
Lane 1 : Fetal brain lysate
Lane 2 : Human glioma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 152 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab137052 被引用在 2 文献中.
- Giannoccaro MP et al. Behaviour and neuropathology in mice injected with human contactin-associated protein 2 antibodies. Brain 142:2000-2012 (2019). PubMed: 31079141
- Patterson KR et al. Mechanisms of Caspr2 antibodies in autoimmune encephalitis and neuromyotonia. Ann Neurol 83:40-51 (2018). PubMed: 29244234