Application
Western blot
Sample
Human Cell lysate - whole cell (Fadu)
Loading amount
30 µg
Specification
Fadu
Treatment
RT8gray for 48hrs+Metformin400mM
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Secondary antibody
Name
Non-Abcam antibody was used: thermo
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/5000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 46 kDa
Positive control
only RT
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 24 2013