为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Cell Biology Apoptosis Intracellular Caspases etc Caspases
使用敲除细胞株进行验证重组RabMAb

重组Anti-Caspase-9抗体[E23] (ab32539)

  • Datasheet
  • SDS
Reviews (5)Q&A (10)References (119)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E23] (ab32539)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody [E23] (ab32539)
  • Flow Cytometry - Anti-Caspase-9 antibody [E23] (ab32539)
  • Immunoprecipitation - Anti-Caspase-9 antibody [E23] (ab32539)
  • Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
  • Flow Cytometry - Anti-Caspase-9 antibody [E23] (ab32539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E23] (ab32539)
  • Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
  • Anti-Caspase-9 antibody [E23] (ab32539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E23] to Caspase-9
  • Suitable for: WB, IHC-P, Flow Cyt, IP, ICC/IF
  • Knockout validated
  • Reacts with: Human

你可能会感兴趣

生物化学
Pifithrin-alpha-HBr, p53 Inhibitor (ab120478)
分析检测
Gaussia Luciferase Cellular Assay Kit (ab189814)
蛋白
Product image
Recombinant human Caspase-9 protein (Tagged) (ab198061)

查看更多相关产品

概述

  • 产品名称

    Anti-Caspase-9抗体[E23]
    参阅全部 Caspase-9 一抗
  • 描述

    兔单克隆抗体[E23] to Caspase-9
  • 宿主

    Rabbit
  • 特异性

    This antibody should recognise both the pro-[40kDa] form and p35 cleaved form of Caspase-9.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide within Human Caspase-9 aa 250-350. The exact sequence is proprietary.
    Database link: P55211

  • 阳性对照

    • WB: Jurkat and HeLa whole cell lysate (ab150035). IHC-P: Human skeletal muscle and cervical carcinoma tissues. ICC/IF: HepG2 cells. Flow Cyt: K562 cells. IP: HeLa whole cell lysate (ab150035).
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    E23
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Cytochrome C
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Cytochrome C
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Alternative Versions

    • Anti-Caspase-9 antibody [E23] - BSA and Azide free (ab219590)
    • PE Anti-Caspase-9 antibody [E23] (ab237431)
  • Assay kits

    • Caspase 9 Assay Kit (Colorimetric) (ab65608)
    • Caspase 9 (active) FITC Staining Kit (ab65615)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant human Caspase-9 protein (Tagged) (ab198061)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
WB
Human
应用 Ab评论 说明
WB (4)
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.

We recommend overnight incubation at 4°C.

IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/100 - 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/80 - 1/100.
ICC/IF
1/500.

For unpurified use at 1/50.

说明
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.

We recommend overnight incubation at 4°C.

IHC-P
1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt
1/100 - 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP
1/80 - 1/100.
ICC/IF
1/500.

For unpurified use at 1/50.

靶标

  • 功能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 组织特异性

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 序列相似性

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 发展阶段

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • 翻译后修饰

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Target information above from: UniProt accession P55211 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 842 Human
    • Omim: 602234 Human
    • SwissProt: P55211 Human
    • Unigene: 329502 Human
    • 别名

      • APAF-3 antibody
      • APAF3 antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase 9 apoptosis related cysteine peptidase antibody
      • Caspase 9 Dominant Negative antibody
      • Caspase 9c antibody
      • Caspase-9 antibody
      • Caspase-9 subunit p10 antibody
      • ICE LAP6 antibody
      • ICE like apoptotic protease 6 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • MCH6 antibody
      • PPP1R56 antibody
      • protein phosphatase 1, regulatory subunit 56 antibody
      • RNCASP9 antibody
      see all

    图片

    • Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
      Western blot - Anti-Caspase-9 antibody [E23] (ab32539)

      Lanes:
      Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
      Lane 2: CASP9 knockout HAP1 whole cell lysate (40 µg)
      Lane 3: HeLa whole cell lysate (40 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32539 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab32539 was shown to specifically recognize Caspase-9 in wild type HAP1 cells along with additional cross-reactive bands. No band was observed when Caspase-9 knockout samples were examined. Wild-type and Caspase-9 knockout samples were subjected to SDS-PAGE. Ab32539 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E23] (ab32539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E23] (ab32539)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Caspase-9 with purified ab32539 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody [E23] (ab32539)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspase-9 antibody [E23] (ab32539)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Caspase-9 with purified ab32539 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Flow Cytometry - Anti-Caspase-9 antibody [E23] (ab32539)
      Flow Cytometry - Anti-Caspase-9 antibody [E23] (ab32539)

      Flow Cytometry analysis of K562 cells labelling Caspase-9 with purified ab32539 at 1/250 (red). Cells were fixed with 100% methanol. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    • Immunoprecipitation - Anti-Caspase-9 antibody [E23] (ab32539)
      Immunoprecipitation - Anti-Caspase-9 antibody [E23] (ab32539)

      ab32539 (purified) at 1/80 immunoprecipitating Caspase-9 in HeLa whole cell lysate.

      Lane 1 (input): HeLa whole cell lysate (10µg)

      Lane 2 (+): ab32539 + HeLa whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32539 in HeLa whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
      Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
      All lanes : Anti-Caspase-9 antibody [E23] (ab32539) at 1/1000 dilution (purified)

      Lane 1 : HeLa whole cell lysate - treated with Camptothecin
      Lane 2 : HeLa whole cell lysate - untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Predicted band size: 46 kDa
      Observed band size: 35,46 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry - Anti-Caspase-9 antibody [E23] (ab32539)
      Flow Cytometry - Anti-Caspase-9 antibody [E23] (ab32539)

      Overlay histogram showing K562 cells stained with unpurified ab32539 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab32539, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was anti-rabbit DyLight® 488 (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E23] (ab32539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E23] (ab32539)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling Caspase 9 with unpurified ab32539 at a dilution of 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
      Western blot - Anti-Caspase-9 antibody [E23] (ab32539)
      All lanes : Anti-Caspase-9 antibody [E23] (ab32539) at 1/20000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate - untreated
      Lane 2 : Jurkat cell lysate - treated with Camptothecin

      Predicted band size: 46 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?
      Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

    • Anti-Caspase-9 antibody [E23] (ab32539)
      Anti-Caspase-9 antibody [E23] (ab32539)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (119)

    发表研究结果有使用 ab32539?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab32539 被引用在 119 文献中.

    • Sun X  et al. Effect of Loureirin B on Crohn's disease rat model induced by TNBS via IL-6/STAT3/NF-?B signaling pathway. Chin Med 15:2 (2020). PubMed: 31911815
    • Duan C  et al. Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways. Cell Death Dis 11:251 (2020). PubMed: 32312970
    • Zhao J  et al. Long non-coding RNA ANRIL down-regulates microRNA-7 to protect human trabecular meshwork cells in an experimental model for glaucoma. Eur Rev Med Pharmacol Sci 23:3173-3182 (2019). PubMed: 31081068
    • Amin AH  et al. Ameliorative effects of melatonin against solid Ehrlich carcinoma progression in female mice. J Pineal Res 67:e12585 (2019). PubMed: 31066091
    • Cui J  et al. WITHDRAWN: Skullcapflavone I has a potent anti-pancreatic cancer activity by targeting miR-23a. Life Sci N/A:N/A (2019). PubMed: 31085241
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 15 Abreviews or Q&A

    Western blot abreview for Anti-Caspase-9 antibody [E23]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U87 cells)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-20%)
    Loading amount
    13 µg
    Treatment
    bevasizumab 0, 1 and 2mg/ml for 24hrs, 48hrs
    Specification
    U87 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 11 2020

    Western blot abreview for Anti-Caspase-9 antibody [E23]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 22 2014

    Western blot abreview for Anti-Caspase 9 antibody [E23]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Fadu)
    Loading amount
    30 µg
    Specification
    Fadu
    Treatment
    RT8gray for 48hrs+Metformin400mM
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 24 2013

    Question


    Can this Anti-Caspase 9 antibody [E23] (ab32539) detects full length caspase-9 or cleaved caspases-9,or both of them?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 06 2013

    Answer


    Full length caspase-9 will cleave into two units, caspase-9 p35 subunit and caspase-9 p10 subunit. The immunogen of ab32539 is a synthetic peptide corresponding to the C terminus of caspase-9 p35 subunit. Therefore, the antibody is able to detect both full length caspase-9 and cleaved caspase-9 p35 subunit.

    Read More

    Abcam Scientific Support

    回复于 Mar 06 2013

    Question

    Please see customers inquiry below:
    I was wondering how effectively these will work on human skeletal muscle biopsy samples. I can’t seem to see an article where these are used under those conditions.

    ab32539

    ab32125

    ab62484

    ab32351

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 06 2012

    Answer

    Thank you for your inquiry.
    I am happy to confirm that all these antibodies are tested and guaranteed to work with human samples.
    You are correct that we do not have any reports yet about the use of these antibodies in muscle. I assume that is your customers area of research. It is not possible for us to test our product with all available tissues and therefore I would like to re-assure you and your customer that these antibodies are covered by our Abpromise guarantee when positive controls beside muscle are used in experiments.
    I would recommend doing this anyway especially if your customer does not know what to expect in muscle as it is part of a research project.
    Maybe your customer would like to consider writing an Abreview after they used the antibodies in their experiments? They would earn Abpoints that can be redeemed a discount on future purchases or Amazon voucher.
    I hope this information is helpful and wish your customer good luck in their experiments.

    Read More

    Abcam Scientific Support

    回复于 Jun 06 2012

    Question

    Suite à relecture de la fiche techinque, je m'appercois qu'il n'est
    pas préconise pour le rat? Ce n'est donc qu'une prédiction par rapport
    à l'homologie n'est ce pas?
    Si vous pensez que ca peut marcher alors nous testons.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 22 2012

    Answer

    La référence ab2014 n'a pas encore été testée chez le rat, c'est pour cela que j'ai préféré comparer les séquences.

    Nous serions ravis si vous pouviez nous communiquer vos résultats. Ceci nous permettra peut-être et je l'espère d'ajouter le rat aux espèces validées pour cet anticorps.

    J'ai donc procédé à l'envoi d'une unité de ab2014 en remplacement du ab32539. Le numéro de la commande est : ******.

    Read More

    Abcam Scientific Support

    回复于 Mar 22 2012

    Question


    L'anticorps qui correspondrait le plus à nos besoin est le ab63488, qui est preconise à la fois pour WB et IHC-P. Les images de WB
    proposées sont un peu limite vous en conviendrait et ne mette pas vraiment l'Ac en valeur. Mais je suppose que ce ne sont pas des essais
    realiser chez abcam?!
    Si vous me confirmer l'utilisation possible de cet anticorps pour WB et IHC-P alors nous le testerons en espérant avoir de meilleur
    resultats que jusqu'à present.
    Dans l'attente de la confirmation

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Merci pour votre réponse.

    L'anticorps ab63488 est en effet garanti pour fonctionner en WB et IHC-P. Je suis d'accord avec vous, l'image du blotn'est pas très attractive.

    Une autre référence qui a retenu mon attentionest ab2014, un polyclonal de lapin, testé WB et ICH-P et dont l'immunogène présente 100%d'homologie avec la protéine derat. J'orienteraispersonnellement mon choixpour cet anticorps, https://www.abcam.com/ab2014

    Merci de m'indiquer ce que vous en pensez.

    Read More

    Abcam Scientific Support

    回复于 Mar 21 2012

    Question

    Monsieur, je reviens vers vous pour
    1/ recommander des anticorps qui fonctionne, demande de devis
    anticaspase-12 ab62484
    anticaspase-3 ab47131
    2/ Par contre pour la caspase-9 nous n'obtenons aucun marquage, hors un anticorps de chez un de vos concurrents nous avez donner dumarquage. Nous voulions simplement regrouper nos achats. Auriez vous un autre anti-corps à nous proposer
    nous avions ab32539 , et avions eu plusieurs problemes que nous avions vu ensemble (dernier mail le 2 Decembre 2011 16:31:09). Nous avons
    tester dilution 1/1000 et 1/500, si vous avez quelque chose à proposer merci de le rajouter au devis et surtout de me transmettre les infos.
    J'attends bien sur geste commerciale conséquent car nous avons investi pour un anticorps qui n'a rien donne! cout des experimentations TRES IMPORTANTS.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 19 2012

    Answer

    Merci de nous avoir contactés de nouveau.

    Je suis vraimentdésolé d'apprendre que le second lot de ab32359 ne fonctionne pas toujours pas comme attendu.

    Afin de solutionnerce problème, je peux vous proposer l'envoi gratuit (puisque le produit est toujours sous garantie) de :
    - soit un autre lot de ab32359 (différent des 2 lots précédemment reçus),
    - soit une unité de ab25758, https://www.abcam.com/ab25758
    - soit une unité de ab63488, https://www.abcam.com/ab63488
    - soit une unité de ab115161, https://www.abcam.com/ab115161
    - soit une unité de ab2013, https://www.abcam.com/ab2013
    - soit une unité de ab47537, https://www.abcam.com/ab47537

    Tous les anticorps anti-caspase9listés ci-dessus sont donnés pour fonctionner en Western Blot et sur échantillons de rat.

    Merci de m'indiquer la résolution qui vous conviendrait le mieux. Bien entendu,l'éditiond'un remboursement oud'un avoir estégalement possible.

    Je vous fais parvenir dans un autre email un devis pour les anticorps ab62484 et ab47131.

    Read More

    Abcam Scientific Support

    回复于 Mar 19 2012

    Question

    I would like to ask what is the isotype of the Anti-Caspase 9 antibody [E23] (ab32539)? Is it IgG1? I found information on the website, that the isotype is IgG, but since it is a monoclonal antibody, I suppose there should also be the number of the subclass.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2012

    Answer

    As far as we know, rabbits do not have specific IgG subclasses like mice or
    humans. All of our antibodies are simply considered to be rabbit IgG.
    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Abcam Scientific Support

    回复于 Feb 07 2012

    Question

    Bonnour, nous avons bien recu le nouvel anticorps mais encore petit probleme puisqu'il y avait une fuite au niveau du bouchon et nous n'avons pu recupéré que 80 µl au lieu de 100 µl! On va quand meme travailler avec ca pour voir si ca marche! Auriez vous une dilution à nous conseiller pour WB?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 02 2011

    Answer

    Je suis vraiment désolé d'apprendre que vous rencontrez un autre problème avec cet anticorps. Pourriez-vous s'il vous plait tester cet anticorps. Si il fonctionne je vous enverrai une nouvelle unité en prenant soin de faire vérifier le tube avant expédition. Si il ne fonctionne pas nous essaierons de trouver ensemble une résolution scientifique ou commerciale. Je recommanderais une dilutiuon au 1/1000 pour cet anticorps. Dans l'attente de vos résultats, je vous souhaite une bonne fin de semaine ainsi qu'un bon week-end.

    Read More

    Abcam Scientific Support

    回复于 Dec 02 2011

    1-10 of 15 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有