重组Anti-Caspase-7抗体[EPR22839-12] (ab255818)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22839-12] to Caspase-7
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Caspase-7抗体[EPR22839-12]
参阅全部 Caspase-7 一抗 -
描述
兔单克隆抗体[EPR22839-12] to Caspase-7 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Untreated wild-type HAP1 whole cell lysate, Wild-type HAP1 treated with 1 M staurosporine for 3 hours whole cell lysate, C6, PC-12, RAW 264.7 whole cell lysate, Untreated HeLa, HeLa treated with 1 M staurosporine for 3 hours whole cell lysate and C2C12 lysate. IHC-P: Human, mouse and rat colon tissues. Flow Cyt (intra): HeLa and C2C12 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22839-12 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab255818于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/50.
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WB |
1/1000. Detects a band of approximately 35, 32, 18 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
1/50. |
WB
1/1000. Detects a band of approximately 35, 32, 18 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
组织特异性
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
序列相似性
Belongs to the peptidase C14A family. -
翻译后修饰
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 840 Human
- Entrez Gene: 12369 Mouse
- Entrez Gene: 64026 Rat
- Omim: 601761 Human
- SwissProt: P55210 Human
- SwissProt: P97864 Mouse
- Unigene: 9216 Human
- Unigene: 35687 Mouse
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别名
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
see all
图片
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All lanes : Anti-Caspase-7 antibody [EPR22839-12] (ab255818) at 1/1000 dilution
Lane 1 : Untreated HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa treated with 1 µM staurosporine for 3 hours whole cell lysate
Lane 3 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 18,32,35 kDa why is the actual band size different from the predicted?Caspase-7 is activated upon apoptotic induction. Procapase-7 (35 kDa), intermediate caspase-7 (32 kDa) and caspase-7 p20 subunit (18 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15953353, 18596415).
Exposure time: Lanes 1-2: 15 seconds; Lane 3: 3 seconds.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Caspase-7 antibody [EPR22839-12] (ab255818) at 1/1000 dilution
Lane 1 : Untreated wild-type HAP1 whole cell lysate
Lane 2 : Wild-type HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
Lane 3 : Untreated caspase-7 knockout HAP1 whole cell lysate
Lane 4 : Caspase-7 knockout HAP1 treated with 1 µM staurosporine for 3 hours whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 32,35 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsCaspase-7 is activated upon apoptotic induction. Procapase-7 (35 kDa), intermediate caspase-7 (32 kDa) and caspase-7 p20 subunit (18 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 15953353, 18596415).
ab255818 was shown to specifically react with Caspase-7 in wild-type HAP1 cells as signal was lost in Caspase-7 knockout cells. Wild-type and Caspase-7 knockout samples were subjected to SDS-PAGE. ab255818 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ instrument using the ECL technique. Blocking and Diluting Buffer and concentration: 5% NFDM/TBST
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C2C12 (mouse muscle) cells labeling Caspase-7 with ab255818 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma) cells labeling Caspase-7 with ab255818 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling Caspase-7 with ab255818 at 1/2000 dilution (0.28 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat colon (PMID: 30448733) is observed. The section was incubated with ab255818 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Caspase-7 with ab255818 at 1/2000 dilution (0.28 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse colon (PMID: 23572575) is observed. The section was incubated with ab255818 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Caspase-7 with ab255818 at 1/2000 dilution (0.28 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon (PMID: 30448733) is observed. The section was incubated with ab255818 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab255818 被引用在 3 文献中.
- Huang B et al. Construction and validation of a prognostic risk model for breast cancer based on protein expression. BMC Med Genomics 15:148 (2022). PubMed: 35787690
- Alekseeva L et al. Human Recombinant DNase I (Pulmozyme®) Inhibits Lung Metastases in Murine Metastatic B16 Melanoma Model That Correlates with Restoration of the DNase Activity and the Decrease SINE/LINE and c-Myc Fragments in Blood Cell-Free DNA. Int J Mol Sci 22:N/A (2021). PubMed: 34769514
- Liu H et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). PubMed: 33356694