Anti-Caspase-7抗体[4D10B2] (ab201959)
Key features and details
- Mouse monoclonal [4D10B2] to Caspase-7
- Suitable for: IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Caspase-7抗体[4D10B2]
参阅全部 Caspase-7 一抗 -
描述
小鼠单克隆抗体[4D10B2] to Caspase-7 -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, Flow Cytmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment corresponding to Human Caspase-7 aa 1-200. (Purified from E.coli).
Database link: P55210 -
阳性对照
- Human Caspase-7 (aa29-198) recombinant protein; Caspase-7 (aa29-198)-hIgGFc transfected HEK293 cell lysate; Jurkat, HEK293, MOLT4, MCF 7 and PC-12 cell lysates; MCF7 cells; Human cervical cancer tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
4D10B2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201959于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200 - 1/1000.
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WB |
1/500 - 1/2000. Predicted molecular weight: 34 kDa.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. Predicted molecular weight: 34 kDa. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
组织特异性
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
序列相似性
Belongs to the peptidase C14A family. -
翻译后修饰
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 840 Human
- Entrez Gene: 64026 Rat
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
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别名
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
see all
图片
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: HAP1 + Staurosporin knockout HAP1 whole cell lysate (20 µg)
Lane 3: CASP7 whole cell lysate (20 µg)
Lane 4: CASP7 + Staurosporin whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: HeLa + Staurosporin whole cell lysate (20 µg)Lanes 1 - 6: Merged signal (red and green). Green - ab201959 observed at 38,20 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab201959 was shown to specifically react with HAP1 + Staurosporin when HAP1 + Staurosporin knockout samples were used. Wild-type and HAP1 + Staurosporin knockout samples were subjected to SDS-PAGE. Ab201959 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-Caspase-7 antibody [4D10B2] (ab201959) at 1/500 dilution + Human Caspase-7 (aa29-198) recombinant protein
Predicted band size: 34 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?Expected MWt is 22.5 kDa.
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All lanes : Anti-Caspase-7 antibody [4D10B2] (ab201959) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Caspase-7 (aa29-198)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 34 kDa -
All lanes : Anti-Caspase-7 antibody [4D10B2] (ab201959) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : PC12 cell lysate
Predicted band size: 34 kDa -
Flow cytometric analysis of MCF7 cells labeling Caspase -7 with ab201959 at 1/200 dilution (green) compared to a negative control (red).
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Caspase-7 with ab201959 at 1/200 dilution.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab201959 被引用在 2 文献中.
- Zierhut C et al. The Cytoplasmic DNA Sensor cGAS Promotes Mitotic Cell Death. Cell 178:302-315.e23 (2019). PubMed: 31299200
- Zhang C et al. Growth of tyrosine kinase inhibitor-resistant Philadelphia-positive acute lymphoblastic leukemia: Role of bone marrow stromal cells. Oncol Lett 13:2059-2070 (2017). WB . PubMed: 28454362