Anti-Caspase-4抗体(ab25898)
Key features and details
- Rabbit polyclonal to Caspase-4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Caspase-4抗体
参阅全部 Caspase-4 一抗 -
描述
兔多克隆抗体to Caspase-4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Caspase-4 aa 20-70 (N terminal).
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阳性对照
- WB: Ramos cell lysate IHC-P: Mouse spleen
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25898于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
Use a concentration of 2 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa). |
IHC-P
Use a concentration of 2 µg/ml. |
靶标
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功能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. -
组织特异性
Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. -
序列相似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻译后修饰
The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. - Information by UniProt
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数据库链接
- Entrez Gene: 837 Human
- Entrez Gene: 12363 Mouse
- Omim: 602664 Human
- SwissProt: P49662 Human
- SwissProt: P70343 Mouse
- Unigene: 138378 Human
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别名
- Apoptotic cysteine protease Mih1/TX antibody
- CASP-4 antibody
- CASP4 antibody
see all
图片
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Lane 1 : Anti-Caspase-4 antibody (ab25898) at 0.5 µg/ml
Lane 2 : Anti-Caspase-4 antibody (ab25898) at 1 µg/ml
All lanes : Ramos cell lysate
Predicted band size: 31 kDa
Observed band size: ~34 kDa why is the actual band size different from the predicted? -
ab25898 Immunohistochemical staining Caspase-4 of mouse spleen using 2 µg/ml (paraffin embedded sections).
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab25898 被引用在 8 文献中.
- Wu Z et al. Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis. Biomolecules 12:N/A (2022). PubMed: 36359010
- Cheng Q et al. Caspase-11/4 and gasdermin D-mediated pyroptosis contributes to podocyte injury in mouse diabetic nephropathy. Acta Pharmacol Sin 42:954-963 (2021). PubMed: 32968210
- Meng J et al. Dendrobine suppresses endoplasmic reticulum stress-induced apoptosis through upregulating microRNA miR-381-3p to decrease caspase-4. Bioengineered 12:4452-4463 (2021). PubMed: 34308746
- Wang X et al. SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade. EMBO J 40:e108080 (2021). PubMed: 34747049
- Kong M et al. miR-512-3p Overcomes Resistance to Cisplatin in Retinoblastoma by Promoting Apoptosis Induced by Endoplasmic Reticulum Stress. Med Sci Monit 26:e923817 (2020). PubMed: 32641679
- Zhang Z et al. Caspase-11-mediated tubular epithelial pyroptosis underlies contrast-induced acute kidney injury. Cell Death Dis 9:983 (2018). PubMed: 30250284
- Ghavami S et al. Apoptosis, autophagy and ER stress in mevalonate cascade inhibition-induced cell death of human atrial fibroblasts. Cell Death Dis 3:e330 (2012). WB ; Human . PubMed: 22717585
- Sansanwal P et al. Caspase-4 may play a role in loss of proximal tubules and renal injury in nephropathic cystinosis. Pediatr Nephrol : (2009). IHC-P ; Human . PubMed: 19705160