Application
Western blot
Sample
Human Cell lysate - whole cell (Triple negative breast cancer)
Gel Running Conditions
Non-reduced Denaturing (15)
Loading amount
10 µg
Treatment
20µM Compound for 24hrs
Specification
Triple negative breast cancer
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 17, 34 kDa Non-specific: 15, 19, 20, 25, 35, 36, 55 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. yushan lin
Verified customer
提交于 Apr 20 2020