Application
Western blot
Sample
Human Cell lysate - whole cell (MOLT4)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Loading amount
100000 cells
Treatment
Bortezomib 0-20hours
Specification
MOLT4
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: CST Anti-Rabbit HRP secondary
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 58 kDa Non-specific: 78, 300 kDa
Positive control
Samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 19 2015