概述

  • 产品名称
    Anti-Caspase-1抗体[EPR19672]
    参阅全部 Caspase-1 一抗
  • 描述
    兔单克隆抗体[EPR19672] to Caspase-1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment within Human Caspase-1 aa 100-300. The exact sequence is proprietary.
    Database link: P29466

  • 阳性对照
    • WB: Untreated THP-1 whole cell lysate; THP-1 treated with 80 nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate; Human lymph node, thymus and spleen lysates.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab207802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/30.
WB 1/1000. Detects a band of approximately 45, 42, 35, 22, 20 kDa (predicted molecular weight: 45, 42, 35, 22, 20 kDa).

靶标

  • 功能
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • 组织特异性
    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • 序列相似性
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • 翻译后修饰
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

图片

  • All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) treated with 80 nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45, 42, 35, 22, 20 kDa
    Observed band size: 20,22,42,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.

  • All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 45, 42, 35, 22, 20 kDa
    Observed band size: 22,35,45 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.

  • ab207802 Immunoprecipitating Caspase-1 in THP-1 (human acute monocytic leukemia) whole cell lysate. 2µg of capture antibody in 0.35mg lysate was used. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/1000 and VeriBlot for IP secondary antibody (HRP) ab131366 was used as the secondary antibody at a dilution of 1/1000.

    Lane 1: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 2: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207802 in THP-1 (human acute monocytic leukemia ) whole cell lysate

    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

文献

This product has been referenced in:
  • Wang Z  et al. Nrf2/HO-1 mediates the neuroprotective effect of mangiferin on early brain injury after subarachnoid hemorrhage by attenuating mitochondria-related apoptosis and neuroinflammation. Sci Rep 7:11883 (2017). Read more (PubMed: 28928429) »
See 1 Publication for this product

客户评价及客户问答

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Application
Western blot
Sample
Human Cell lysate - whole cell (LX-2 Human Hepatic Stellate Cell Line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
LX-2 Human Hepatic Stellate Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 Jun 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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