Anti-CASC5/KNL1抗体(ab70537)
Key features and details
- Rabbit polyclonal to CASC5/KNL1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CASC5/KNL1抗体
参阅全部 CASC5/KNL1 一抗 -
描述
兔多克隆抗体to CASC5/KNL1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human CASC5/KNL1 aa 300-400. Synthetic peptide corresponding to Human CASC5/KNL1. Database link: NP_733468.2
Database link: Q8NG31 -
阳性对照
- Whole cell lysate from HeLa or 293T cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately >300 kDa (predicted molecular weight: 265 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately >300 kDa (predicted molecular weight: 265 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Four known isoforms exist. -
细胞定位
Acrosome. Nucleus. -
数据库链接
- Entrez Gene: 57082 Human
- Omim: 609173 Human
- SwissProt: Q8NG31 Human
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别名
- Protein D40/AF15q14 antibody
- AF15Q14 antibody
- ALL1 fused gene from chromosome 15q14 antibody
see all
图片
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All lanes : Anti-CASC5/KNL1 antibody (ab70537) at 1/5000 dilution
Lane 1 : Whole cell lysate from 293T cells at 50 µg
Lane 2 : Whole cell lysate from 293T cells at 15 µg
Developed using the ECL technique.
Predicted band size: 265 kDa
Observed band size: >300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
1mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70537 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot, 20% of the precipitate was loaded per lane, and ab70537 was used at 1ug/ml. Detection: chemiluminescence with exposure time of 3 minutes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab70537 被引用在 12 文献中.
- Germanova TE et al. Subcellular Euclidean distance measurements with multicolor fluorescence localization imaging in cultured cells. STAR Protoc 2:100774 (2021). PubMed: 34841272
- Cordeiro MH et al. Kinetochore phosphatases suppress autonomous Polo-like kinase 1 activity to control the mitotic checkpoint. J Cell Biol 219:N/A (2020). PubMed: 33125045
- Zhou C et al. The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I. Sci Adv 6:eaax3969 (2020). PubMed: 32284991
- Roscioli E et al. Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors. Cell Rep 31:107535 (2020). PubMed: 32348762
- Vallardi G et al. Division of labour between PP2A-B56 isoforms at the centromere and kinetochore. Elife 8:N/A (2019). PubMed: 30829571