Anti-Carbonic Anhydrase 9/CA9抗体(ab184006)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 9/CA9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Carbonic Anhydrase 9/CA9抗体
参阅全部 Carbonic Anhydrase 9/CA9 一抗 -
描述
兔多克隆抗体to Carbonic Anhydrase 9/CA9 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Carbonic Anhydrase 9/CA9 aa 1-168. The exact sequence is proprietary.
Database link: Q16790 -
阳性对照
- HepG2 cell lysate, treated with 200µM CoCl2 for 24hr; Human cervical carcinoma tissue; CoCl2- treated A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184006于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100 - 1/1000.
|
|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
组织特异性
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
序列相似性
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
翻译后修饰
Asn-346 bears high-mannose type glycan structures. -
细胞定位
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
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别名
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution.
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All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate, untreated
Lane 2 : HepG2 whole cell lysate, treated with 200 µM CoCl2 for 24 hr
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa10% SDS PAGE
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Immunofluorescent analysis of A431 cells labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution. A431 cells
were fixed in ice-cold MeOH for 5 min. Cells were treated with 200 μM CoCl2 (right) or untreated (left)
Green: Carbonic Anhydrase 9/CA9 protein showing membrane staining.
Blue: Hoechst 33342.
实验方案
数据表及文件
-
SDS download
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Datasheet download
文献 (12)
ab184006 被引用在 12 文献中.
- Bess SN et al. Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy. BMC Immunol 23:20 (2022). PubMed: 35461243
- Qorri B et al. Repositioning of Old Drugs for Novel Cancer Therapies: Continuous Therapeutic Perfusion of Aspirin and Oseltamivir Phosphate with Gemcitabine Treatment Disables Tumor Progression, Chemoresistance, and Metastases. Cancers (Basel) 14:N/A (2022). PubMed: 35892853
- Grgic I et al. Tumor Oxygenation by Myo-Inositol Trispyrophosphate Enhances Radiation Response. Int J Radiat Oncol Biol Phys 110:1222-1233 (2021). PubMed: 33587991
- Wang Q et al. Combined transcriptomic and lipidomic analysis reveals aberrant lipid metabolism in central nervous system hemangioblastomas. Sci Rep 11:1314 (2021). PubMed: 33446752
- Chen XJ et al. A novel lymphatic pattern promotes metastasis of cervical cancer in a hypoxic tumour-associated macrophage-dependent manner. Angiogenesis 24:549-565 (2021). PubMed: 33484377