重组Anti-Carbonic anhydrase 2/CA2抗体[EPR5195] - BSA and Azide free (ab222480)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5195] to Carbonic anhydrase 2/CA2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Carbonic anhydrase 2/CA2抗体[EPR5195] - BSA and Azide free
参阅全部 Carbonic anhydrase 2/CA2 一抗 -
描述
兔单克隆抗体[EPR5195] to Carbonic anhydrase 2/CA2 - BSA and Azide free -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HAP1, A431, HEK293, HEK293T, and Caco-2 cell lysates, and Mouse brain, Mouse heart, Rat brain, Rat spleen, Rat kidney, and Human heart tissue lysates. IHC-P: Human colon, Rat kidney, Mouse kidney, and Human clear cell carcinoma tissues.
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常规说明
ab222480 is the carrier-free version of ab124687.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解离常数(KD)
KD = 5.42 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5195 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab222480于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
说明 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
靶标
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功能
Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. -
疾病相关
Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation. -
序列相似性
Belongs to the alpha-carbonic anhydrase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 760 Human
- Entrez Gene: 12349 Mouse
- Entrez Gene: 54231 Rat
- Omim: 611492 Human
- SwissProt: P00918 Human
- SwissProt: P00920 Mouse
- SwissProt: P27139 Rat
- Unigene: 155097 Human
see all -
别名
- CA 2 antibody
- CA II antibody
- CA-II antibody
see all
图片
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This data was developed using ab124687, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Carbonic anhydrase 2/CA2 knockout HAP1 cell lysate (40 µg)
Lane 3: A431 cell lysate (40 µg)
Lane 4: HEK293 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124687 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.ab124687 was shown to specifically react with Carbonic anhydrase 2/CA2 when Carbonic anhydrase 2/CA2 knockout samples were used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. Ab124687 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
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This data was developed using ab124687, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Carbonic anhydrase 2/CA2 with purified ab124687 at 1/1600 (0.063 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
All lanes : Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/5000 dilution (Purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat spleen lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaThis data was developed using ab124687, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CA2 knockout HEK293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab124687).
Lanes 1-4: Merged signal (red and green). Green - ab124687 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab124687 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab124687 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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This data was developed using ab124687, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Carbonic anhydrase 2/CA2 with purified ab124687 at 1/1600 (0.063 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
All lanes : Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/5000 dilution (Purified)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Human heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaThis data was developed using ab124687, the same antibody clone in a different buffer formulation.
-
This data was developed using ab124687, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Carbonic anhydrase 2/CA2 with purified ab124687 at 1/1600 (0.063 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
This data was developed using ab124687, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma tissue sections labeling Carbonic anhydrase 2/CA2 with purified ab124687 at 1/1600 (0.063 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (1)
ab222480 被引用在 1 文献中.
- Gorrieri G et al. Goblet Cell Hyperplasia Requires High Bicarbonate Transport To Support Mucin Release. Sci Rep 6:36016 (2016). ICC/IF ; Human . PubMed: 27786259