Anti-Cannabinoid Receptor II抗体(ab45942)

概述

  • 产品名称
    Anti-Cannabinoid Receptor II抗体
    参阅全部 Cannabinoid Receptor II 一抗
  • 描述
    兔多克隆抗体to Cannabinoid Receptor II
  • 宿主
    Rabbit
  • 特异性
    According to BLAST results, the antibody could cross-react with both rat isoforms. No experiments were done to confirm this possibility.
  • 经测试应用
    适用于: IHC-FoFr, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Rat Cannabinoid Receptor II.

    (Peptide available as ab45941.)

  • 阳性对照
    • ab45942 gave a positive result in the following tissue lysates: Rat Spinal Cord, and Mouse Thymus.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab45942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 45 kDa).Can be blocked with Cannabinoid Receptor II peptide (ab45941).
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能
    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • 组织特异性
    Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰
    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • 细胞定位
    Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all

图片

  • Immunocytochemical immunofluorescence analysis of methanol-fixed HT29 human cell line, labelling cannabinoid receptor II with ab45942 at 1/50 dilution incubated for 18 hours at 4°C in 1% BSA. Fixed cells were permeabilized with 0.25% Tween. Secondary used was a Goat anti-Rabbit polyclonal Alexa Fluor® 488. Counterstain is DAPI against nuclear DNA.

    See Abreview

  • All lanes : Anti-Cannabinoid Receptor II antibody (ab45942) at 1 µg/ml

    Lane 1 : Rat spinal cord tissue lysate
    Lane 2 : Mouse thymus tissue lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 8 minutes
  • ab45942 staining Cannabinoid Receptor II in Rat ileum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked with 10% BSA for 10 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody for 17 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab45942 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45942, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Elbaz M  et al. Novel role of cannabinoid receptor 2 in inhibiting EGF/EGFR and IGF-I/IGF-IR pathways in breast cancer. Oncotarget 8:29668-29678 (2017). Read more (PubMed: 27213582) »
  • Martín-Saldaña S  et al. Spontaneous Cannabinoid Receptor 2 (CB2) Expression in the Cochlea of Adult Albino Rat and Its Up-Regulation after Cisplatin Treatment. PLoS One 11:e0161954 (2016). Read more (PubMed: 27564061) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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