Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (pituitary, skin, adrenal)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
pituitary, skin, adrenal
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: VectorLab
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
No visible or convincing membrane staining in any of the tissue types I've used (pituitary gland, adrenal gland, and skin), in IHC-P or IHC-Fr. So far, whether this antibody works in Rhesus Macaque tissues is inconclusive.
Abcam response
Abcam has not tested this antibody in Rhesus macaque, therefore we are unable to guarantee its performance in this species. We thank the researcher for their review, and welcome any further feedback in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 07 2020